Nov 19, 2016 · $\begingroup$ "There is an upper limit to Kcat / Km, imposed by the rate at which E and S can diffuse together in an aqueous solution. This diffusion controlled limit is 10^8 to …

Aug 12, 2020 · The [S] terms cancel, which is why at saturating conditions, Vo = Vmax. Since kcat is a decent surrogate for Vmax here, what this means is that kcat tells us roughly how well the …

Aug 22, 2016 · Kcat = turnover of substrate to product / unit time. Kcat/Km = measure of catalytic efficiency. You use this if the question is asking about which enzyme has the greatest …

Jul 9, 2019 · Kcat does not change for competitive inhibition because [E]t is maintained with enough substrate, that is to say that a high enough [S] will boot inhibitors out of the active site. …

Jun 11, 2017 · Kcat is the rate of reaction of the enzyme; turnover of substrate to product / unit time. Vmax = Kcat [Enzyme] I should probably mention, you most likely will not see any Kcat …

May 25, 2021 · The definition of catalytic efficiency is kcat/Km. So to have a high catalytic efficiency, you want a large numerator (large kcat) and/or a small denominator (small Km). …

Jul 30, 2020 · Aside from grammar and punctuation fixes, your question needs attention to the following issues: 1. Please mention the source of the information (kcat remaining unchanged …

May 17, 2017 · Kcat is how fast the enzyme is at producing product from substrate. Hill coefficient ( 1 is the middle): measures cooperative binding. Below 1 is negative cooperativity, where as …

Sep 30, 2020 · Hi I wrote KCAT on September 2023, my mark was KCAT level 2. All I can suggest is to study jurisprudence as much as you can. Unfortunately, the resources presented …

Oct 9, 2020 · What we do know, is that regardless of mechanism, Km will include all of the microscopic k values (substrate binding, substrate dissociating, etc) up to and including the …