Here, we report the development of a second generation WASF3 mimetic peptide, WAHMIS-2, which was designed using a combination of structure-guided design, homology modeling, and in silico optimization to disrupt binding of WASF3 to the WRC.

Feb 2, 2016 · Activation of the WASF3 protein by extracellular stimuli promotes actin cytoskeleton reorganization and facilitates cancer cell invasion, whereas WASF3 depletion suppresses invasion and metastasis.

WASF3, a Wiskott–Aldrich syndrome protein family member, appears to play a major role not only in the regulation of actin cytoskeleton dynamics but also in cancer cell invasion/metastasis.

First, we show that STAT3 binds directly to the WASF3 promoter and increases transcription levels, which correlates with increased migration potential. Inactivation of STAT3 with short hairpin RNA, dominant negative constructs or S3I-201 leads to …

Feb 2, 2021 · WASF3 (WAVE3), a Wiskott–Aldrich syndrome protein family member, appears to play a major role not only in the regulation of actin cytoskeleton dynamics but also in cancer cell invasion/metastasis. Blocking the WASF3-dependent metastatic signaling network remains an attractive and promising therapeutic option for the treatment of advanced tumors. 1.

Wiskott-Aldrich syndrome protein family members (WASF) regulate the dynamics of the actin cytoskeleton, which plays an instrumental role in cancer metastasis and invasion. WASF1/2/3 forms a hetero-pentameric complex with CYFIP1/2, NCKAP1/1 L, Abi1/2/3 and BRK1 called the WASF Regulatory Complex (WRC …

To investigate the effects of deleting WASF3 in spontaneous models of breast cancer metastasis, a conditional Wasf3 null mouse strain was generated and it was shown that homozygous inactivation of Wasf3 leads to viable mice with no observable …

Sep 1, 2016 · In this study, we demonstrate that HER2/HER3 facilitates WASF3 phospho-activation and promotes WASF3 transcription through JAK2 activation of STAT3 in response to NRG. Loss of WASF3 leads to attenuated epithelial-to-mesenchyme transition (EMT) and invasion induced by NRG.

Dec 12, 2011 · Using oligonucleotide arrays we now demonstrate that knockdown of WASF3 leads to the upregulation of the KISS1 metastasis suppressor gene with concomitant reduced invasion and loss of MMP9 activity. Using a luciferase reporter, KISS1 transcription is significantly increased in the absence of WASF3.

Jan 15, 2021 · WASF3, a Wiskott–Aldrich syndrome protein family member, appears to play a major role not only in the regulation of actin cytoskeleton dynamics but also in cancer cell invasion/metastasis.