Pair powerful whole transcriptome spatial gene expression with single cell–scale resolution to discover the unique biology in every micron of tissue. Obtain best-in-class data with the simple-to-run CytAssist-enabled workflow. Manufactured under license from Centrillion.

Apr 19, 2024 · With a novel slide architecture and a Visium CytAssist–enabled workflow, Visium HD delivers best-in-class, high-resolution spatial transcriptomics data from human or mouse fresh frozen (FF), fixed frozen, and FFPE samples, enabling powerful discoveries like those already demonstrated in our v1 and v2 Visium assays.

Visium Spatial Gene Expression is a next-generation molecular profiling solution for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context in FFPE or fresh frozen tissues to discover novel insights into normal development, disease pathology, and clinical translational research.

We have now updated Seurat to be compatible with the Visium HD technology, which performs profiling at substantially higher spatial resolution than previous versions. Users can install the Visium HD-compatible release from Github.

The Visium HD assay allows for whole-transcriptome spatial gene expression discovery in human or mouse tissue (Figure 1). The workflow begins with sectioning tissue onto glass slides, tissue staining, and imaging. Next, tissues are incubated with either human or mouse probes, which allows the probes to hybridize to target mRNAs.

Building on the 10x Visium Digital Spatial Gene Expression kit, Visium HD has exciting new features that open spatial assay horizons. Map the whole transcriptome within the tissue context at high-definition 2um resolution.

Mar 26, 2024 · Combining the power of whole transcriptome spatial discovery with histological insights, Visium HD enables comprehensive characterization of FFPE tissue samples.

Mar 29, 2024 · Visium HD is designed to allow users to comprehensively characterize FFPE tissue sections by measuring whole transcriptome spatial gene expression at single cell-scale resolution.

Proper tissue handling and preparation techniques preserve the morphological quality of the tissue sections and the integrity of mRNA transcripts. This is critical for downstream library preparation and generation of high quality sequencing data using the Visium Spatial Gene Expression protocols.

Our Visium v1/v2 and Visium HD product lines offer distinct slide architecture and multiple chemistries to explore the whole transcriptome spatial biology of your samples at the resolution you need.