Dec 17, 2018 · To identify the brain areas that participate in generating the VTA Vglut2-mediated wakefulness, we mapped cFos activity in VTA Vglut2-hM3Dq mice.

Oct 4, 2022 · Ventral tegmental area (VTA) is one of main regions with DA-producing neurons. VTA DAergic projections in mesolimbic brain regions play a crucial role in regulating anxiety-like behaviors,...

Jun 30, 2022 · In the VTA, cells activated (cFOS-positive) by stress predominantly expressed the GABAergic marker Vgat (60%) or GABA (57%) (Fig. 1I and fig. S5, A and B), whereas relatively fewer cells expressed the glutamatergic marker Vglut2 (20%) or the dopaminergic marker tyrosine

Jan 9, 2024 · By cFos quantification, we found that VTA optical stimulation of LH-VGluT2 fibers induced local cFos expression in ChR2-eYFP mice (Supplementary Fig. 2L, M).

VTA GABAergic neurons produce a profound sedative state when artificially activated (Yu et al., 2018), but VTA dopaminergic (VTA DA) and VTA Vglut2 cells are selectively wake-and REM-active during normal sleep (Eban-Rothschild et al., 2016).

Numbers of TH+/c-Fos+ neurons in the substantia nigra (SN) were not significantly different across groups, whereas those in the ventral tegmental area (VTA) were significantly different and greatest in PS recovery.

Dec 1, 2012 · In the present experiments we hypothesized that a food-associated CS (light), which demonstrably functions as such by eliciting conditioned responses (CRs), comes to acquire the capacity to activate VTA DA neurons. In Experiment 1, rats were allowed to eat or not eat food (food being the US).

Our results show that focal injections of DAMGO in the VTA increases cFos expression in the majority of its projecting areas, namely NAc, basolateral amygdala (BLA), cingulate cortex (ACC) and bed nucleus of the stria terminalis (BNST), as compared to aCSF.

Jul 1, 2022 · Transient stress enhances the activity of VTAVgat-Sst cells for several hours, allowing them to exert their sleep effects persistently. Lesioning of VTAVgat-Sst cells abolished SDS-induced sleep; without it, anxiety and corticosterone …

Aug 1, 2017 · Immunohistochemical analysis showed increased cFos expression in the VTA after CNO injection in animals from the VTA-NAcc group (Fig. 1 h), which was significantly higher compared to the Sham and VTA-mPFC groups (Fig. 1 i).