This vector allows for constitutive Cre and shRNA expression. It does not contain any selection cassette. The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites.

U6 is the most optimal promoter to produce shRNAs, while CMV is used to express whole genes. Best. Pol III genes and promoters do not get polyadenylated and thus are ideal for making small...

The BLOCK-iT™ Inducible H1 RNAi Entry Vector Kit and the BLOCK-iT™ U6 RNAi Entry Vector Kit (Catalog nos. K4920-00 and K4945-00, respectively) use a vector-based approach to allow efficient generation of RNAi cassettes for constitutive or regulated expression of shRNA molecules in mammalian cells.

U6 is a type III RNA polymerase III promoter commonly used for driving small hairpin RNA (shRNA) expression in vector-based RNAi. In the design and construction of viral vectors, multiple transcription units may be arranged in close proximity in a space-limited vector.

Nov 19, 2004 · Short hairpin RNAs (shRNAs) have been used to achieve stable target knockdown in a variety of biological systems. Here, we report the development of a tightly regulated tetracycline-responsive human U6 promoter for shRNA expression.

We have designed an shRNA-expressing construct controlled by a Pol III U6 promoter to silence a mutant Cu,Zn superoxide dismutase (SOD1 G93A) allele that causes amyotrophic lateral sclerosis (ALS), a fatal degenerative motor neuron disease .

Using mRNA encoding reporter firefly luciferase as a model target, we compared the performance of a schistosome and a human promoter from the U6 gene in driving shRNA in human fibrosarcoma cells and in cultured schistosomes. Further, both a retroviral (Murine leukemia virus [MLV]) and plasmid (piggyBa c, pXL-Bac II) vector were utilized.

Results: ShRNA expression from the human U6 promoter resulted in a fourfold increase in knockdown efficiency compared to expression from the murine U6 promoter in both human and murine cells. Conclusions: The U6 promoter constitutes an important determinant for efficient gene silencing by shRNAs.

Nov 19, 2004 · Short hairpin RNAs (shRNAs) have been used to achieve stable target knockdown in a variety of biological systems. Here, we report the development of a tightly regulated tetracycline-responsive human U6 promoter for shRNA expression.

Plasmid pAAV.U6.shRNA-Sdc1-1376 from Dr. Kevin Park's lab contains the insert shRNA, eGFP and is published in Neuron. 2019 Aug 21;103 (4):642-657.e7. doi: 10.1016/j.neuron.2019.05.044. Epub 2019 Jun 26. This plasmid is available through Addgene.