Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. [5] In eukaryotes, the introns in the transcribed pre-mRNA first have to be removed by spliceosome in order to form a mature mRNA. A spliceosome is assembled from small nuclear ribonucleoproteins (snRNP) and small nuclear RNAs (snRNA).

Mar 30, 2025 · This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of …

May 20, 2022 · The essential pre-mRNA splicing factor U2AF2 (also called U2AF65) identifies polypyrimidine (Py) tract signals of nascent transcripts, despite length and sequence variations. Previous studies have shown that the U2AF2 RNA recognition motifs (RRM1 and RRM2) preferentially bind uridine-rich RNAs.

Mar 4, 2025 · U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing.

Jun 11, 2021 · U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an essential pre-mRNA splicing factor in an early step of splicing. Alternative splicing plays an important role in neuronal...

Feb 7, 2023 · U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an indispensable pre-mRNA splicing factor in the early process of splicing. Recently, U2AF2 was reported as a novel candidate gene...

Nov 3, 2024 · U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing.

Gene ID: 22185, updated on 8-Feb-2025. Enables C2H2 zinc finger domain binding activity. Involved in RNA splicing. Predicted to be located in nucleus. Predicted to be part of Prp19 complex; U2-type spliceosomal complex; and U2AF complex. Predicted to be active in …

High-throughput sequencing of hematologic malignancies and other cancers has revealed recurrent mis-sense mutations of genes encoding pre-mRNA splicing factors. The essential splicing factor U2AF2 recognizes a polypyrimidine-tract splice-site signal and initiates spliceosome assembly.

Patient cells showed a 50% reduction in wildtype U2AF2 mRNA and protein expression (65-kD) compared to controls. A smaller 55-kD band corresponding to expression of the abnormal protein with the in-frame deletion was detected.