Purchase high quality cryopreserved human malignant glioblastoma multiforme (GBM) U251 cells from ECACC that contain cancer stem cells for brain cancer research

In the present study, we aimed to clarify the validity of using U251 glioma cells as a source of GSC culture and critically evaluate the heterogeneous stem-like phenotypes of these cells when grown under various culture conditions.

HLA antigens of established human cell lines.

Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (ECACC catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251.

In the present study, we aimed to clarify the validity of using U251 glioma cells as a source of GSC culture and critically evaluate the heterogeneous stem-like phenotypes of these cells when grown under various culture conditions.

We propose that in U251 cells p53 R273H exerts a regulatory effect on gene transcription by acting as DNA/chromatin associated factor that participates in the organization of chromatin into functional domains.

U-251 MG Glioblastoma Cell Line that expresses EGFR and contains GFAP positive cells. Buy Here!

Here, we developed a novel method for the 3D cultivation of the human glioblastoma cell line U251 within the nanofibrillar cellulose hydrogel, GrowDex®. We demonstrate that 3D cultivation of U251 cells in GrowDex results in an increased cell viability.

Methods: We studied the MGMT-methylated GBM cell line U251 and its in vitro derived TMZ-resistant subline, U251/TMZ-R. Cytotoxicity of TMZ, CCNU, and radiation was tested. Both cell lines were analyzed for MGMT promotor status and expression of mismatch repair genes (MMR).

The present study established a novel method for obtaining CD133 (+) and CD133 (−) U251 cells. In the current study, gene recombination technology was successfully used to construct two types of gene expression vectors, which were stably transfected in the U251 cell line.