U11 and U12 snRNPs bind U12-type pre-mRNAs as a preformed di-snRNP complex, simultaneously recognizing the 5' splice site and branchpoint sequence. Thus, within the U12-type prespliceosome, U11/U12 components form a molecular …

The U11 snRNA (small nuclear ribonucleic acid) is an important non-coding RNA in the minor spliceosome protein complex, which activates the alternative splicing mechanism. The minor spliceosome is associated with similar protein components as the major spliceosome.

Feb 6, 2025 · In the structure of the substrate-bound U11 snRNP, we uncovered an unexpected bipartite recognition mechanism, wherein the 5′SS proximal region of the intron (positions +3 and +4) bind to the U11 snRNA SL3, while the distal positions (+5 to +11) bind to the 5′ end of the U11 snRNA, as expected.

Feb 12, 2025 · The Galej Group at EMBL Grenoble has provided new insights into minor spliceosome function, revealing the structure of U11 snRNP – one of its subunits, which initiates the intron selection process.

Dec 22, 2023 · We obtained a cryo-EM reconstruction of the 13-subunit U11 snRNP complex, revealing structures of U11 snRNA and five minor spliceosome-specific factors. U11 snRNP appears strikingly different from the equivalent major spliceosome U1 snRNP.

U11 and U12 snRNPs bind U12-type pre-mRNAs as a preformed di-snRNP complex, simultaneously recognizing the 5′ splice site and branchpoint sequence. Thus, within the U12-type prespliceosome, U11/U12 components form a molecular …

Sep 7, 2005 · We demonstrate that the U11/U12-65K protein possesses dual binding activity, interacting directly with U12 snRNA via its C-terminal RRM and the U11-associated 59K protein via its N-terminal half.

Native gel electrophoresis reveals that U11, U12, and U5 snRNPs assemble onto the P120 pre-mRNA to form splicing complexes. Inhibition of P120 splicing by 2′-O-methyl oligonucleotides complementary to U12 or U5 demonstrates that U12 and U5 snRNPs perform essential roles in the AT–AC spliceosome.

We have identified the cDNA encoding this protein by microsequencing a 14 kDa protein isolated from U2-type spliceosomes. This protein contains an RNA recognition motif and is highly conserved across species.

Aug 2, 2024 · SNRNP25 and SNRNP35 specifically recognize U11 snRNA, while PDCD7 bridges SNRNP25 and SNRNP48, located at the distal ends of the particle. SNRNP48 and ZMAT5 are positioned near the 5' end of U11 snRNA and stabilize binding of the incoming 5'SS.