A bright fluorescent dye that can be used in microarray applications. TYE 563 can be used as a direct substitute for Cy3®.

1 fluorescent transfection control DsiRNA (TYE 563-labeled transfection control) Nuclease-Free Duplex Buffer IDT recommends that the qPCR assay be located close to site of the DsiRNA target sites to avoid measuring a cleaved transcript that has a remaining fragment.

TYE dyes are proprietary fluorophores from IDT that are available for commonly used dye wavelengths. The numerical value in the name of these dyes represents the emission wavelength when attached to an oligo. TYE 563: A bright fluorescent dye that can be used in microarray applications. TYE 563 can be used as a direct substitute for Cy 3.

The TYE 563 Transfection Control DsiRNAs and HPRT-S1 Positive Control DsiRNAs are included in TriFECTa Kits to assist with this phase of the RNAi project. Cationic lipids are commonly used to transfect DsiRNAs into tissue culture cells.

The TYE-563 fluorescent transfection control duplex (cat# [SR30002]) indicates that >90% of the cells have been transfected; The HPRT positive control (cat# [SR30003]) provides 90% knockdown efficiency.

Shop Integrated DNA Technologies Inc TYE 563 TRANSFECTION CNTRL at Fishersci.com

TYE 563 + + 549: 563: Cy3: TEX 615 + + 596: 613: Texas Red-X 617: TYE 665 + 645: 665: Cy5: TYE 705 + 686: 705 : TET + 522 539 : HEX + 538: 555 : Fluorescein dT + + 495: 520 Rhodamine dyes 5'-end Int 3'-end Excit Emit Alternative; TAMRA + 559: 583 : TAMRA NHS Ester + + + 559: 583 : ROX NHS Ester + + 588: 608 : Other 5'-end Int 3'-end Excit Emit

Nov 18, 2019 · The rehydrated cells are probed with the reference strain (0.1 μM) probe (TYE 563) in 30% formamide (Sigma) and 2 × SSC overnight. This allows the microscope to stabilize at room temperature ...

siTran 2.0 was used to co-transfect TYE-563 labeled siRNA (CAT# SR30002) and GFP plasmid DNA (CAT# PS100093). Images were taken 48 hrs post transfection.

Apr 4, 2018 · Both the bronchioli and alveolar epithelia of mice treated with VIPER polyplexes show an increased amount of TYE™-563 siRNA in the bronchi and alveolar epithelium when compared to the lung tissue of mice treated with CP polyplexes (Figure 8B). It was therefore hypothesized that VIPER polyplexes efficiently reach the cytoplasm of bronchial and ...