TurboGFP is a basic (constitutively fluorescent) green fluorescent protein published in 2006, derived from Pontellina plumata. It is reported to be a rapidly-maturing dimer with moderate acid sensitivity.

Improved green fluorescent protein from Pontellina plumata. Sticky ends from different BseRI sites may not be compatible. BseRI quickly loses activity at 37°C. Prolonged incubation with BseRI may lead to degradation of the DNA. ApaI can be used between 25°C and 37°C. Sticky ends from different BanII sites may not be compatible.

TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.

Aug 25, 2006 · We produced a highly soluble, rapidly maturing variant, named TurboGFP. Having solved its crystal structure, we were able to analyse the chromophore environment and explain the rapid development of the fluorescent signal in vivo.

We have used 50ns molecular dynamics simulations of the mature and immature forms of avGFP and TurboGFP to examine the diffusion of water molecules in-and-out of the protein β-barrel. Most crystal structures of GFPs have well-structured waters within hydrogen-bonding distance of Glu222 and Arg96.

TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.

Vector for expressing TurboGFP in bacteria. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. Sticky ends from different BbsI sites may not be compatible. BbsI gradually loses activity when stored at -20°C. Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.

TurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation. Main properties of TurboGFP Characteristic

TurboGFP exhibits extremely fast proteinfolding kinetics and chromophore maturation, with high protein solubility (Evdokimov et al., 2006). Thus, it was unsurprising that the small hydrophobic ...

In this report, we find that GFP affects T cell activation, leading to defects in clustering, upregulation of the activation marker CD25 and IL-2 cytokine production following stimulation in human primary T cells that also express TurboGFP.