Tet-On 3G is a modified, improved Tet-On Advanced transcriptional activator protein (transactivator) which has been evolved to display far higher sensitivity to doxycycline. Tet-On 3G generates very high maximal expression and responds to lower Dox …

May 8, 2017 · Here, we report the use of the PiggyBac transposon and a Tet-On 3G drug-inducible gene expression system to achieve versatile inducible gene expression in hPSC lines. Our new system, XLone, offers...

The Tet-On 3G Systems are inducible gene expression systems for mammalian cells. Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox) (Figure 1). Figure 1.

Tet-On 3G is the gold standard tetracycline-inducible expression system for mammalian cells, generating the lowest basal expression and highest fold-induction.

Plasmid AAVS1-TRE3G-EGFP from Dr. Su-Chun Zhang's lab contains the insert EGFP and is published in Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653. This plasmid is available through Addgene.

Typical applications are listed here. The Tet-On 3G system, combines an optimized tetracycline-dependent reverse transactivator with an improved responsive promoter. The Tet-On 3G transactivator, activates expression of responsive genes in the presence of the inducing agent (e.g. doxycycline, Dox).

Oct 18, 2023 · HEK cells were transduced to stably expressing a pTRE3G-mCherry reporter, HSP-hyperdCas12a-miniVPR for transcriptional activation and guide RNA crTet targeting the TRE3G promoter.

Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver 3–4 orders of magnitude difference in expression levels between induced and uninduced states. These systems are available in lentiviral-, retroviral-, …

The Tet-On 3G protein (TRE3G), which contains 5 amino acid distinctions from M2rtTA2, further increases the sensitively to dox and decreases the background expression when used in combination with optimized tetracycline response element (TRE) repeats [15].

Here, we report the use of the PiggyBac transposon and a Tet-On 3G drug-inducible gene expression system to achieve versatile inducible gene expression in hPSC lines. Our new system, XLone, offers improvement over previous Tet-On systems with significantly reduced background expression and increased sensitivity to doxycycline.