TPY Broth is a medium recommended for Bifidobacterium. The genus Bifidobacterium is the third most numerous bacterial population in the human intestine after the genera Bacteroides and Eubacterium. It is also the predominant population during childhood.

Selective medium for the isolation of Bifidobacterium

Tryptone soya yeast extract broth ISO 11290-2:1998 NutriSelect® Plus, suitable for microbiology; Synonyms: TSYEB at Sigma-Aldrich

After two successive transfers in TPY broth at 37°C for 24 h under anaerobic condition using Anaerocult A gas packs (Merck, Darmstadt, Germany), the activated culture was then properly diluted and served as the standard inoculum for all cultivations.

Gibco™ Tryptose Phosphate Broth is a cell culture supplement for BHK-21 cells used for vaccine production. Tryptose Phosphate Broth contains tryptose (an enzymatic hydrolysis of protein), dextrose, sodium chloride and disodium phosphate.

Technical data for TPY Broth, a selective medium for Bifidobacterium isolation. Includes formula, preparation, uses, and testing.

Selective Medium for the isolation of Bifidobacterium. Suspend 36 grams of the medium in one liter of distilled water .Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.

TPY Broth Usages: For cultivation of bifidobacteria and determination of fructose-6-phosphate phosphatase transketolase (F6PPK). Principle:

USAGE : For cultivation of bifidobacteria and determination of fructose-6-phosphate phosphatase transketola

Growth of bifidobacteria was tested in trypticase-phytone-yeast extract (TPY; Becton-Dickinson) broth (2) and in a mixture of IST broth (90%) and MRS broth (10%) adjusted to pH 6.7 and supplemented with l -cysteine hydrochloride (0.3 g liter −1 and 0.5 g liter −1; Sigma).