May 17, 2012 · We show that coexpression of murine JAK2 V617F and the murine thrombopoietin (Tpo) receptor (TpoR, c-MPL) in hematopoietic cell lines or heterozygous knock-in of JAK2 V617F in mice leads to down-modulation of TpoR levels.

Nov 19, 2024 · Live-cell micropatterning experiments confirmed the promiscuous binding of JAK1, JAK2, and TYK2 to IFNAR1 and IFNAR2, and their recruitment correlated with their relative cellular abundances. However, each JAK had a different efficacy in inducing cross-phosphorylation and downstream signaling.

The thrombopoietin receptor (TpoR) regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. TpoR signals by activating Janus kinases JAK2 and Tyk2.

Nov 23, 2023 · In this issue of Blood, Papadopoulos et al 1 reveal that oncogenic JAK2V617F elicits a unique pattern of active human thrombopoietin receptor (hTpoR) conformations distinct from those induced by its cognate ligand thrombopoietin (Tpo).

Feb 29, 2016 · mCALR mutants activate the JAK-STAT pathway and enable cytokine-independence of Ba/F3 – TpoR cells.

Dec 15, 2006 · We showed that the cytosolic membrane-proximal region of the TpoR stabilizes the mature receptor isoform from degradation, and is both necessary and sufficient for its high level surface expression in the absence of JAK2.

JAK signaling globally counteracts heterochromatic gene silencing in a hematopoietic tumor model which elicits the de-repression of non-STAT targets. For example, constitutive JAK activation promotes the suppression of heterochromatin-mediated …

Here we show that, in addition to signaling downstream from the activated TpoR, JAK2 and Tyk2 strongly promote cell surface lo-calization and enhance total protein levels of the TpoR. This effect is caused by stabilization of the mature en-doglycosidase H-resistant form of the receptor.

Jul 22, 2005 · The thrombopoietin receptor (TpoR) regulates hematopoietic stem cell renewal, megakaryocyte differentiation, and platelet formation. TpoR signals by activating Janus kinases JAK2 and Tyk2.

Dec 15, 2006 · Janus tyrosine kinase 2 (JAK2) is essential for signaling by the thrombopoietin (TpoR) and erythropoietin (EpoR) receptors. In the absence of JAK2 most EpoR molecules are retained in the endoplasmic reticulum in an Endo H-sensitive form.