The transcriptionally active fragment of the yeast RNA polymerase II transcription elongation factor, TFIIS, comprises a three-helix bundle and a zinc ribbon motif joined by a linker region. We have probed the function of this fragment of TFIIS using structure-guided mutagenesis.

Transcription elongation factor A protein 1 is a protein that in humans is encoded by the TCEA1 gene. [5][6][7] In other organisms, this gene is better known as transcription elongation factor II S (TFIIS). It mainly helps to resolve backtracked elongation complexes by inducing a cut in the RNAP active site, so reaction becomes possible again.

Here, we used a mutant version of the tran-scription elongation factor TFIIS (TFIISmut), aiming to specifically induce increased levels of RNAPII pausing, arrest, and/or backtracking in human cells.

Inducing transcription stress via TFIIS mut results in reduced nascent RNA synthesis. TFIIS mut affects expression of long genes and mRNA splicing. TFIIS mut expression leads to increased R-loop formation and genomic instability.

Jan 30, 2023 · TFIIS is an elongation factor that stimulates the transcript cleavage activity of RNAPII to reactivate stalled elongation complexes at barriers to transcription including nucleosomes.

Mar 20, 2014 · Using optical-trapping technology, we performed a single-molecule study of RNAPII interactions with transcription factors TFIIS and TFIIF, which are involved in modulating and regulating transcriptional elongation.

Transcription elongation by RNA polymerase II is regulated by the general elongation factor TFIIS. This factor stimulates RNA polymerase II to transcribe through regions of DNA that promote the formation of stalled ternary complexes.

The transcriptionally active fragment of the yeast RNA polymerase II transcription elongation factor, TFIIS, comprises a three-helix bundle and a zinc ribbon motif joined by a linker region. We have probed the function of this fragment of TFIIS using structure-guided mutagenesis.

Oct 9, 2007 · In this article, we provide direct evidence that the evolutionarily conserved transcription elongation factor TFIIS functions during preinitiation complex assembly. First, we identified TFIIS in a mass spectrometric screen of RNA polymerase II (Pol II) preinitiation complexes (PICs).

Jan 31, 2022 · Elongation factor TFIIS (transcription factor IIS) is structurally and biochemically probably the best characterized elongation cofactor of RNA polymerase II. However, little is known about TFIIS regulation or its roles during stress responses.