Compared to their predecessors (Tet-On and Tet-On Advanced systems), Tet-On 3G systems demonstrate lower basal expression (by 5- to 20-fold) and higher sensitivity to doxycycline …

Tet-On 3G is the gold standard tetracycline-inducible expression system for mammalian cells, generating the lowest basal expression and highest fold-induction.

The first step in establishing the Tet-On 3G System in your cells is creating a stable cell line that: (1) expresses the Tet-On 3G transactivator; (2) demonstrates high levels of induction from PTRE3G ; and (3) exhibits low basal expression from PTRE3G.

Based on the transcriptional regulators described by Gossen and Bujard, 1992; Gossen et al. 1995; and Urlinger et al. 2000; Tet-On 3G is a modified form of the Tet-Advanced transactivator protein which has been altered to display far higher sensitivity to doxycycline (Dox) (Zhou et …

The Tet-On 3G Systems are inducible gene expression systems for mammalian cells. Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G or PTRE3GV) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox) (Figure 1).

Discover Tet-On 3G inducible gene expression: learn about the technology, applications, choosing the right system for your application, and available products.

Tet-On 3G cell lines greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. These rigorously tested clonal cell lines stably express our premium Tet-On 3G transactivator.

Lenti-X Tet-On 3G inducible expression systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system.

All plasmid-based versions of the Tet-On 3G System contain the following seven components: 10 μg regulator plasmid (see tables below) 10 μg response plasmid (see tables below) 10 μg control plasmid expressing luciferase under the control of a TRE3G promoter (either pTRE-Luc or …

This page lists the remaining 2nd generation Tet-On and Tet-Off vectors (Tet-Advanced). To learn about the highest-performing 3rd generation Tet-On inducible expression systems, visit our Tet-On 3G and Tet-One pages.