Plasmid pLKO-Tet-On-shRNA-Control from Dr. Roland Friedel's lab contains the insert non-targeting randomized sequence and is published in Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7.

Titration of the inducing agent (Dox or Tet), as well as determination of the optimum incubation period to achieve maximal target knock- down, is recommended for each individual target gene.

The SMARTvector Inducible Lentiviral shRNA vectors incorporate the Tet-On® 3G bipartite induction system, a third-generation Tet-inducible system significantly improved and optimized for minimal basal expression (lowest leakiness) and potent …

Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). [1]

Here, we describe a method to efficiently generate stable human Tet-on cell lines that reliably drive either inducible overexpression or depletion of the gene of interest.

Dec 13, 2007 · Here we describe a simple and rapid protocol for the generation of tetracycline (Tet)-inducible vectors that express shRNAs in a time- and dosage-dependent manner.

The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic biological research to biotechnology and gene therapy applications.

Together, the Tet-On 3G protein and TRE3G promoter permit tight regulation of the shRNA expression, including potent induction, even at the low doxycycline doses that are required in vivo .

The doxycycline (dox)-inducible Tet-On system is widely used to control gene expression in mammalian cells. This system is based on the bacterial Tet operon, which has been modified and improved for its function in eukaryotic cells.

Discover Tet-On 3G inducible gene expression: learn about the technology, applications, choosing the right system for your application, and available products.