The TOPO™ TA Cloning™ Kits for Sequencing provide a highly efficient, 5-minute, one-step cloning strategy ('TOPO™ Cloning') for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing.

The TA TOPO cloning technique relies on the ability of adenine (A) and thymine (T) (complementary base pairs) on different DNA fragments to hybridize and, in the presence of ligase or topoisomerase, become ligated together.

TOPO TA Cloning provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO Cloning") for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required.

TOPO® TA Cloning® Kit for Subcloning (Cat. no. 450641) is shipped with only the TOPO® TA Cloning® reagents (Box 1). TOPO® TA Cloning® Kits are available with pCRTM 2.1-TOPO® or pCRTM II-TOPO® vector.

TA, also known as sticky-end, TOPO cloning exploits the inherent activity of Taq polymerase, which adds a single deoxyadenosine (A) to the 3'-end of PCR products. When inserts are amplified by PCR with Taq polymerase, the product can …

This protocol describes the use of TOPO-activated TA vectors for cloning. Manufacturers of cloning kits provide excellent manuals that explain in detail what to do and why to do it.

TOPO TA cloning is a better alternative to the conventional (ligation-based) TA cloning method. This article discusses the principle, advantages, and limitations of TOPO TA cloning.

The TOPO™ TA Cloning™ Kits for Sequencing provide a highly efficient, 5 minute, one-step cloning strategy ('TOPO™ Cloning') for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing. Supplier: Invitrogen™ K457501.

Oct 27, 2016 · Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form hydrogen bonds.