TAp63-mediated senescence overrides Ras-driven transformation of p53-deficient cells, preventing tumour initiation, and doxycycline-regulated expression of TAp63 activates p21 Waf/Cip1, induces senescence and inhibits progression of established tumours in vivo.

All isoforms of TAp63 transactivate the p21-encoding gene, with TAp63β showing the highest levels of p21 induction (Helton et al., 2008). The transcriptional activation of p21 by TAp63 requires both the AD and proline-rich domains of TAp63.

We found that downregulation of p53, p63 and TAp63 decreases the expression of growth-suppressing genes, including p21, PUMA and MIC-1. Interestingly, downregulation of p63, particularly TAp63, but not p53, disrupts the ability of MDCK cells to form a cyst structure in 3-D culture via heightened epithelial-to-mesenchymal transition (EMT).

TAp63-mediated senescence overrides Ras-driven transformation of p53-deficient cells, preventing tumour initiation, and doxycycline-regulated expression of TAp63 activates p21(Waf/Cip1), induces senescence and inhibits progression of established tumours in vivo.

Apr 3, 2025 · Additionally, we evaluated the impact of the miR-205-5p mimic on TAp63 downstream genes, including P21, BAX, and DICER. The results indicated a significant reduction in BAX and P21 expression in ...

Nov 28, 2018 · p53, with its family members p63 and p73, have been shown to promote myoblast differentiation by regulation of the function of the retinoblastoma protein and by direct activation of p21 Cip/Waf1 and p57 Kip2, promoting cell cycle exit.

Apr 1, 2013 · We found that like downregulation of p53, downregulation of p63 and TAp63 decreases expression of growth-suppressing genes, including p21, PUMA and MIC-1, and consequently promotes cell...

Thus, TAp63 has an activity similar to p53, such as in inducing p21 [3]. ΔNp63 lacks the activation domain conserved in p53 but carries a unique activation domain in the N-terminus, which is composed of 14 unique N-terminal residues and the proline-rich domain [4].

Jul 6, 2005 · The expression of TAp63α was functional, as shown by the ability to induce expression of p21. Following the induction of p21, the cells showed a G1 cell cycle arrest, with a significant reduction in S and G2/M phases, as indicated in Figure 3B.

Consistent with NIR acting as a repressor, the induced translocation of NIR from the nucleolus into the nucleoplasm resulted in the inhibition of TAp63-dependent transactivation of p21. Conversely, knockdown of NIR by RNAi stimulated p21 transcription in the presence of TAp63.