Optimization of tamoxifen-induced Cre activity and its effect
Sep 17, 2020 · In addition to establishing optimal dose and administration of TAM, our study reveals a disparate activity of Cre in different cell immune populations when using Cre/ER …
Intraperitoneal Injection of Tamoxifen for Inducible Cre-Driver Lines
For adult mice, a standard dose of 100µl tamoxifen/corn oil solution (above) is effective for inducing recombination. Administer tamoxifen via intraperitoneal injection (using an ACUC …
Optimizing tamoxifen-inducible Cre/loxp system to reduce tamoxifen …
For tamoxifen-dependent Cre recombinase, also known as CreER recombinase, tamoxifen (TAM) is used to activate the Cre to generate time- and tissue-specific mouse mutants. TAM is a …
Tamoxifen induction of Cre recombinase does not cause long …
The systemic delivery of tamoxifen (Tam) to activate inducible CreERT2-loxP transgenic mouse systems is now widely used in neuroscience studies. This critical technological advancement …
A review of tamoxifen administration regimen optimization for Cre…
Sep 1, 2023 · Tamoxifen-induced Cre/loxp system. Without tamoxifen, Cre-ER exhibits binding affinity for HSP90 and localizes to the cytoplasm. Upon tamoxifen injection into mice, it …
Tamoxifen-Induced Cre-loxP Recombination Is Prolonged in …
The advent of tamoxifen (Tm)-inducible Cre recombinases has greatly improved the ability to temporally control Cre-loxP recombination in vivo, and has been particularly useful for …
Optimization of tamoxifen-induced Cre activity and its effect ... - PubMed
Sep 17, 2020 · Tamoxifen (TAM) inducible Cre recombinase system is an essential tool to study gene function when early ablation or overexpression can cause developmental defects or …
Specificity and efficiency of tamoxifen-mediated Cre induction is ...
Dec 15, 2023 · Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (<2 …
Tamoxifen-Activated CreERT Impairs Retinal Angiogenesis …
Jul 8, 2020 · Endothelial Cre recombinase estrogen fusion protein with ligand binding mutation (CreER) T2 activity impairs retinal angiogenesis. Flat mounted perinatal day (P) 7 retinas were …
Specificity and efficiency of tamoxifen-mediated Cre induction is ...
Nov 9, 2023 · Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (<2 …