TZM-bl cells are permissive to infection by a wide variety of HIV-1, SIV, and SHIV strains, including primary HIV isolates and molecularly cloned Env-pseudotyped viruses.

The TZM-bl indicator cell line enables quantitative analysis of HIV infection using either β-gal or luciferase as a reporter. The parental cell line (JC.53) stably expresses large amounts of CD4 and CCR5 and constitutively express CXCR4.

Neutralizing Antibody Assay for HIV-1 in TZM-bl Cells (October 2021) Introduction This assay measures neutralization in TZM-bl cells as a function of a reduction in Tat-induced. uciferase (Luc) reporter gene exp. ession after a single round of virus infection. TZM-b.

The TZM-bl assay measures antibody-mediated neutralization of HIV-1 as a function of reductions in HIV-1 Tat-regulated firefly luciferase (Luc) reporter gene expression after a single round of infection with Env-pseudotyped viruses. This assay has ...

TZM-bl is an adherent cell line that is maintained in T-75 culture flasks. Cell monolayers are disrupted and removed by treatment with trypsin/EDTA at confluency when splitting cells for routine maintenance and when preparing cells for assay.

The parent TZM-bl cell line is an engineered HeLa cell clone that expresses human CD4, CCR5 and CXCR4 and contains HIV-1 T. Perez LG, et al. J. Virol. 83:7397-7410, 2009.

Jan 22, 2022 · The HeLa cell-derived TZM-bl reporter cell line is widely used for the estimation of lentiviruses neutralization by immune sera. The cell line is highly permissive to infection by most strains of HIV, SIV, and SHIV.

Sep 1, 2024 · TZM-bl cells, a highly susceptible cell line to HIV-1 due to their high CD4 and CXCR4 receptor expression, are crucial in HIV research. They are employed in luciferase reporter assays for measuring viral replication, neutralization assays to evaluate antibody efficacy, and drug susceptibility testing.

Jan 7, 2022 · The cell line is highly permissive to infection by most strains of HIV, SIV, and SHIV. Here we demonstrated that TZM-bl cells express a 48 kDa non-glycosylated protein (p48) recognized by broadly neutralizing monoclonal antibody (mAb) 2F5 targeting the ELDKWA (aa 669–674) epitope of gp41TM of HIV-1.

The TZM-bl indicator cell line enables simple and quantitative analysis of HIV using either -gal or luciferase as a reporter. It is maximally sensitive to HIV infection by including DEAE-dextran in …