The PCDNA3.1 and PCDNA3.1-TRPV1 plasmids were transfected in 293 cells to construct stable cell lines termed 293/PCDNA3.1 and 293/PCDNA3.1-TRPV1, respectively. qPCR confirmed that the...

Dec 11, 2020 · In the membrane separation experiment, we transfected HEK 293T cells with the same amount (3 μg) of TRPV1 cDNA in all four groups, whereas the amount of Kvβ1 cDNA was adjusted to achieve a molar ratio of TRPV1 and Kvβ1 at 1:1 and 1:2, respectively.

Jun 21, 2018 · We identified TRPV1 variants in two patients and their heterologous expression in muscles of trpv1−/− mice strongly enhanced calcium release from SR upon halogenated anesthetic stimulation,...

Jan 15, 2025 · To verify that the highly expressed TRPV1 receptors on DA neurons mediate the anchoring of ATB NPs to the cell membrane, we transfected human embryonic kidney (HEK) 293T cells with a TRPV1 expression plasmid and incubated the cells with ATB NPs (1 …

Jun 14, 2022 · Histamine promotes a direct binding of H1R to TRPV1 via TRPV1 deSUMOylation, which enhances H1R-TRPV1 functional coupling in sensory neurons and itching behaviors. Disrupting the H1R-TRPV1 interaction with blocking peptides suppresses histaminergic itch …

Aug 4, 2020 · Exposure of TRPV1-ferritin-expressing HEK-293T cells at 30 °C to an alternating magnetic field of 501 kHz and 27.1 mT significantly increased SEAP secretion by ~ 82% relative to control...

To test the effects of oxytocin on TRPV1 channels, initial experiments used a heterologous expression system, Human Embryonic Kidney (HEK)-293 cells, to stably express the channel. Oxytocin concentrations used were in the low micromolar range, which matches some physiological conditions.

In order to assess whether TRPV1 plays a role in responses to a broad array of substances, TRPV1 (along with green fluorescent protein) was expressed in human embyonic kidney cells (HEK) 293t cells which were then stimulated with diverse trigeminal irritants.

TRPV1 channels support the detection of noxious and nociceptive input. Currently available functional and structural data suggest that TRPV1 channels have two gates within their permeation...