To reprogramme the resistance response of N towards bacterial pathogens, the helicase domain (p50) of the TMV replicase, the avirulence gene of N, was linked to synthetic promoters 4D …

We demonstrate that recombinant p50 protein has ATPase activity, as suggested by the presence of conserved sequence motifs found in ATPase/helicase enzymes. A point mutation that alters …

Apr 30, 2002 · In this mini review we discuss recent advances in the understanding of the N gene-mediated resistance to tobacco mosaic virus (TMV). The tobacco N gene belongs to toll …

Jan 5, 2013 · To study N gene activity in seeds and early seedling development, the avirulence gene of N, the helicase domain (p50) of the TMV replicase, was constitutively expressed in a …

The results pointed to three novel features. First, the N factor was found to directly bind to the p50 of TMV, second, ATP was pre-requisite for this interaction, with formation of an ATP/N factor …

Jan 5, 2002 · Like the HR elicited by TMV infection, transgenic expression of p 50 induces a temperature-sensitive defence response. We demonstrate that recombinant p 50 protein has …

Feb 8, 2008 · NRIP1 interacts directly with N's TIR domain and TMV's p50 effector in yeast two-hybrid analyses and is the first protein identified that interacts with both a TIR-NB-LRR …

Feb 13, 2007 · In tobacco and its relatives, the N resistance protein confers resistance to infection by the Tobacco mosaic virus (TMV). We have used N, and the TMV elicitor, p50, to investigate …

Jun 3, 2013 · (b) Upon TMV infection, p50 binds first indirectly to N's TIR domain, then directly to the NB and LRR domains, leading to conformational changes and oligomerization of the N …

Dec 30, 2005 · The sequence of the P50 elicitor (nucleotides 2082 to 3418 from TMV, which correspond to the helicase domain of the viral replicase) (Erickson et al., 1999) was amplified …