In this study we used TMV to test whether a near atomic resolution structure can be obtained using a commercially available 4k × 4k CCD camera with an ultra sensitive scintillator to collect cryo-EM images at 300 keV. TMV is a highly ordered plant virus.

Tobacco mosaic virus[a] (TMV) is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family Solanaceae. The infection causes characteristic patterns, such as "mosaic"-like mottling and discoloration on the leaves (hence the name).

EMD-2833 - Cryo-EM structure of TMV reconstructed from micrographs recorded with the K2 Summit DED

Mar 29, 2019 · Here, we present a microfluidic method for the rapid isolation of a target protein and its direct preparation for cryo-EM. Less than 1 μL of cell lysate is required as starting material to solve the atomic structure of the untagged, endogenous human 20S proteasome.

Jan 20, 2007 · We have made improvements to this approach using Tobacco Mosaic Virus (TMV) as a test specimen and obtained a map from 210,000 asymmetric units at a resolution better than 5 A. This was made possible by performing a full correction of the contrast transfer function of …

Here, we present two cryo-EM structures of the helical TMV assembly at 2.0 and 1.9 Å resolution in conditions of high Ca 2+ concentration at low pH and in water.

Sep 19, 2019 · Capsid structures of viruses need to be disassembled for releasing the viral genome. This study presents 1.9/2.0 Å‐resolution cryo‐ EM structures of the helical plant virus TMV explaining the TMV disassembly switch mechanism.

Sep 1, 2010 · Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with...

Jan 1, 2018 · Recently, TMV single-particle 3D reconstructions to 3.2 Å resolution have been achieved by cryo-electron microscopy (cryo-EM) using direct electron detectors confirming that fiber diffraction and cryo-EM yield equivalent data (Fromm et al., 2015).

Feb 1, 2015 · Here, we provide a comparative case study of highly ordered TMV recorded on different direct electron detectors to establish recording and processing conditions that enable structure determination up to 3.2 Å in resolution using cryo-EM.