In THP-1 cells, LPS has been shown to induce the production of pro-inflammatory cytokines such as interleukin-1β (IL-1β), tumour necrosis factor (TNF-α) and interleukin-6 (IL-6) via the TLR4- NFκB signaling pathway [12,13].

The lipopolysaccharide (LPS) of Gram-negative bacteria is recognized on human monocytes and macrophages by TLR4 and MD2 and induces the production of inflammatory cytokines; the LPS + IgG complexes co-stimulation increases the cytokine production, mediated by …

Time dependent cytokine secretion was investigated to study the inflammation-related responses at protein level. LPS stimulation induced inflammation-related cytokine, COX-2 and NF-κB genes of THP-1 monocytes and THP-1 macrophages with the …

Here, we provide a dataset for validated protocol when THP-1 cells are differentiated into LPS inducible macrophages by using phorbol-12-myristate-13-acetate (PMA).

Oct 1, 2021 · We combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate spatiotemporal proteomic changes during a lipopolysaccharide (LPS)-induced inflammatory response.

Sep 27, 2018 · THP-1 cells pre-incubated with LPS for 2–48 h had a significantly reduced ability to release the pro-inflammatory cytokines tumor necrosis factor alpha (TNFα) and interleukin (IL)-8 in response to a second stimulation with LPS (100 ng/ml) (Figure 1B).

Oct 10, 2016 · THP-1 is a human monocytic cell line derived from an acute monocytic leukaemia patient. THP-1 cells are notably sensitive to LPS and respond by expressing many inflammatory cytokines. In the...

May 25, 2022 · In this study, we investigated the differences in susceptibility to the effects of LPS between THP-1 cells, human monocytic cell line and phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells. We also examined the changes in activation of MAPKs and NF-κB pathway according to LPS sensitivity in these cells.

These studies examine the molecular basis for increased transcription of tissue factor (TF) in THP-1 cells stimulated with lipopolysaccharide (LPS). DNase I footprinting identified six sites of protein-DNA interaction between −383 and the cap site …

Sep 1, 2006 · Our data demonstrates that LPS stimulates THP-1 cell adhesion at LPS concentrations ranging from 0.01 to 10 μg/ml, indicating LPS-stimulated adhesion can occur below concentrations that are required for direct LPS-β2 integrin interactions.