We demonstrate that CD8 + or CD4 + T cells in patients with cancer can be molecularly defined using a combination of spectratyping (TCR structure and “molecular composition”) plus the implementation of an antibody panel directed against 21 individual VB TCR chains (“quantity” of …

The TCR V beta repertoire varies significantly between individuals and populations because of 7 frequently occurring inactivating alterations (ie, polymorphisms) in functional gene segments and a large deletion/insertion-related alteration encompassing 2 V beta gene segments.

T-cell receptor (TCR)-Vβ repertoire analysis is a sensitive method for detection of T-cell clonality. This type of analysis has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity against microbial and …

Spectratyping is a broadly utilized technique to measure global TCR diversity by the analysis of the lengths of CDR3 fragments in each Vβ family.

We combined analysis of TCR-VB frequency, determined by flow cytometry and the TCR-VA/VB CDR3 length measurement in individuals undergoing vaccination with recombinant protein.

Apr 30, 2021 · T-cell receptor (TCR)-Vβ repertoire analysis is a sensitive method for detection of T-cell clonality. This type of analysis has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity...

We demonstrate that CD8(+) or CD4(+) T cells in patients with cancer can be molecularly defined using a combination of spectratyping (TCR structure and "molecular composition") plus the implementation of an antibody panel directed against 21 individual VB TCR chains ("quantity" of …

Our results suggest that a differential expression of particular TCR-Vb families and high levels of circulating CD4+ CD25high T cells in long-term surviving renal transplant patients could contribute to an active and specific state of immunologic suppression.

In the present study, TCR-Vb repertoires of cancer patients were primarily analyzed by flow cytometry. Abnormally expressed TCR-Vb subfamilies were generally found in both TIL and peripheral blood lymphocytes (PBL) of each patient.

By use of primers specific for human T-cell receptor (Tcr) Vb6 gene elements, a typing system for Tcr Vb variability in several artiodactyl species has been established. The amplified polymorphic locus is homologous to the human Vb6 gene element.