T-cell receptor (TCR) stimulation causes the dephosphorylation of NFAT which in almost every kind of T cell then forms a complex with AP-1 (except in Tregs). This complex depending on …

Jun 1, 2005 · In T cells, NFAT proteins are activated following T-cell receptor (TCR) ligation. Increases in calcium that are induced by TCR engagement activate calcineurin, which causes …

Functional endpoint measures of TCR stimulation are determined by measuring either cytokine productions (IFNγ or IL2) or measuring activation of a reporter construct driven by a Nuclear …

Jun 23, 2023 · Here, we show that two key pathways in T cell signaling, calcium/nuclear factor of activated T cells (NFAT) and protein kinase C (PKC)/nuclear factor κB (NF-κB), integrate …

Pathway integration is further regulated temporally, where T cell receptor (TCR) and chimeric antigen recep-tor (CAR) activation signals modulate the temporal relationships between the …

Nef-mediated inhibition of NFAT signalling activity was assessed by transfecting the Nef clones into a Jurkat T cell line, stimulating the TCR with anti-CD3 antibody, and then measuring the …

A cornerstone of the adaptive immune response is the T cell receptor (TcR)-calcium-calcineurin signalling pathway leading to T cell activation. The 'nuclear factor of activated T cells' proteins …

Feb 21, 2013 · NFAT dimers induce the transcription of genes involved in T cell tolerance (Soto-Nieves et al., 2009), whereas NFAT interaction with other DNA-binding factors that require …

T cell activation following antigen binding to the T cell receptor (TCR) involves the mobilization of intracellular Ca 2+ to activate the key transcription factors nuclear factor of activated T …

The T Cell Activation Bioassay (NFAT) is a bioluminescent cell-based assay that overcomes the limitations of existing assays and can be used for the discovery and development of novel …