A Practical Approach to T-Cell Receptor Cloning and Expression
We show that a recombination-based cloning protocol facilitates simple and rapid transfer of the TcR transgene into different expression systems. The presented comprehensive method can …
Identifying and cloning the T cell receptor - Nature
Dec 6, 2022 · T cells are able to detect and respond to specific peptide antigens by virtue of the unique T cell receptor (TCR) complexes they express on their cell surface (see image).
Rapid cloning of antigen-specific T-cell receptors by ... - Nature
Apr 7, 2022 · We used the detection-and-cloning system to clone a tumour-antigen-specific TCR from peripheral blood mononuclear cells of healthy donors. TCR cloning by leveraging the cis …
TCR sequencing and cloning methods for repertoire analysis and ...
We also present a TCR cloning strategy to specifically amplify TCRs from T cell populations. Positioned downstream of single-cell or bulk TCR sequencing, it allows time- and cost …
T-ISAAC: a novel TCR cloning system derived from T cells’ cis ...
Jul 6, 2022 · In a recent article published in Nature Biomedical Engineering, Kobayashi and colleagues revealed a novel T cell activation induced by cis interaction, and developed the T …
Revealing Clonal Responses of Tumor-Reactive T-Cells Through T …
In this review, we introduce two novel approaches for immunological interpretation of the TCR repertoire. Inter-organ clone tracking analysis based on bulk TCRseq can enrich tumor …
scRNA+TCR-seq Reveals the Proportion and Characteristics of Dual TCR …
1 day ago · Figure 1. Characterizes single Treg TCR and dual TCR Treg in the Blood, Kldney, Liver, LPL, and Pancreas of mice. A, Mouse source names, GEO accession number, total …
TCR transgenic clone selection guided by immune receptor ... - eLife
Dec 30, 2024 · Implementing newly created DALI software for TCR repertoire analysis in single-cell analysis enabled the rapid selection of the ideal responder CD8 T cell clone, based on …
The T cell receptor landscape of childhood brain tumors
Mar 19, 2025 · A TCR-seq analysis revealed the presence of full-length TCR sequences in approximately half of the T cells (Fig. 3D; ... Analysis of abundant T cell clones demonstrated …
We also present a TCR cloning strategy to spe-cifically amplify TCRs from T cell populations. Positioned downstream of single-cell or bulk TCR sequencing, it allows time- and cost …