As was the case for Apaf1, induction of TAp73 was significantly reduced by treatment with TCDD (Figure 4 A), suggesting that the interaction of activated AHR with E2F1 suppresses the transactivation of proapoptotic E2F1 target genes.

3.3. AhR/E2F1. AhR can also directly interact with E2F1, which transactivates genes essential not only for cell cycle progression, but also for promoting apoptosis. In particular, cellular stress by DNA damage results in phosphorylation of E2F1 by checkpoint kinase-2 (CHK2).

Feb 1, 2024 · In this study, we used transcriptomics data from the BaP- and TCCD-exposed human liver cell line HepG2, and performed differential eigengene network analysis to understand the correlation among genes and to untangle the common regulatory mechanism in the action of BaP and TCDD.

In summary, we identified a consensus transcriptional regulatory network common for BaP and TCDD consisting of direct AHR targets and AHR-TF targets. This analysis sheds new light on the common mechanism of action of a genotoxic (BaP) and …

Oct 10, 2011 · Our results showed that E2F1 mRNA was significantly downregulated in response to BaP and TCDD, which mechanism might further contribute to the inhibition of apoptosis in prostate cells with significant levels of BPDE-DNA adducts.

Feb 2, 2017 · Comparative genome-wide gene expression analysis identified a limited number of candidate orthologs deregulated by TCDD in all three species and a large number of divergent and species-specific responses elicited by TCDD across human, mouse and rat primary B cells.

Jun 23, 2010 · TCDD-mediated activation of Ahr pathway in proliferating T lymphocytes leads to an increased apoptosis in E2F2 −/− T cells. T cells were activated using anti-CD3 antibody for 36 hours and subsequently treated with TCDD for the referred times.

Using a conditional gene-targeting approach, we show that the targeted disruption of the entire E2F activator subclass composed of E2f1, E2f2, and E2f3 in mouse embryonic fibroblasts leads to the activation of p53 and the induction of p53 target genes, including p21CIP1.

Jan 13, 2021 · AhR can also directly interact with E2F1, which transactivates genes essential not only for cell cycle progression, but also for promoting apoptosis. In particular, cellular stress by DNA damage results in phosphorylation of E2F1 by checkpoint kinase-2 (CHK2).

Feb 17, 2017 · Here, through single-cell quantitative analysis, we demonstrate that E2F1 expression levels have a critical role in determining the fate of individual cells. Low levels of exogenous E2F1...