T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5'→ 3' direction. [1]

Nov 10, 2022 · For many research and commercial applications, IVT of mRNA is typically performed using bacteriophage T7 RNA polymerase (T7 RNAP) owing to its ability to produce …

Mar 16, 2023 · The resulting biocatalyst can now be applied to the streamlined manufacture of mRNA products with reduced immunogenicity, addressing a key challenge in the development …

Most commonly used in vitro translation systems utilize T7 RNA polymerase, which is also the enzyme included in many commercial kits. Here we present characterization of a variant of T7 …

T7 RNA polymerase is responsible for beginning transcription at the T7 promoter of the transformed vector. The T7 gene is itself under the control of a lac promoter. Normally, both …

Jun 20, 2024 · Therapeutic messenger RNA (mRNA) has been demonstrated as a scalable and versatile vaccine platform for the rapid development and manufacture of new vaccine …

Aug 14, 2020 · In vitro transcription using T7 bacteriophage polymerase is widely used in molecular biology. Here, we use 5′RACE-Seq to screen a randomized initially transcribed …

To overcome these challenges, we have engineered a novel RNA polymerase, T7-68, that co-transcriptionally incorporates both di- and tri-nucleotide cap analogs with high efficiency, even …

Dec 1, 2024 · T7 RNA Polymerase (T7 RNAP) is a high-fidelity enzyme used for the synthesis of single-stranded mRNA from DNA templates using In vitro transcription (IVT). T7 RNAP is now …

Apr 9, 2025 · The mMESSAGE mMACHINE™ T7 mRNA Kit with CleanCap™ Reagent AG utilizes this technology to generate high yields of mRNA (>5 mg/mL) with over 95% capping …