T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second …

Oct 8, 2019 · T7 endonuclease I (T7EI) mismatch detection assays1 are commonly employed to evaluate the gene editing efficiency of CRISPR-Cas9 reagents at a given guide RNA target site in a population of edited cells.

The T7 endonuclease I (T7EI) mismatch cleavage assay detects on-target genome editing and estimates genome editing efficiency in CRISPR-treated cells. Straightforward—use standard molecular biology techniques; Fast—does not require purification of PCR products before analysis; Consistent—analyze easy-to-read electrophoresis results

Jan 17, 2018 · In this study, we explored the accuracy of four assays (T7E1, TIDE, IDAA and NGS) frequently used to determine the level of activity for a given sgRNA when complexed with Cas9. We compared the...

T7 endonuclease I (T7EI) mismatch cleavage assay for detection of on-target editing, known off-target events, and estimation of genome editing efficiency in cultured cells. Learn more

Endonucleases such as endonuclease T7 can be powerful tools in determining targeting efficiency and off-target mutations as long as their advantages and limitations are considered. Bloom, K., Ely, A., & Arbuthnot, P. (2016). A T7 Endonuclease I Assay to Detect Talen-Mediated Targeted Mutation of HBV cccDNA.

The GeneArt Genomic Cleavage Detection Kit is a fast, T7 endonuclease I based method to quantify how well your genome editing protocol causes insertions and deletions (indels) in the genome of your cell line.

T7 Endonuclease I (T7E1) Assay Protocol for CRISPR/Cas9 gRNA validation Genomic DNA primer design 1 Design genomic DNA primers that are approximatley 18 to 22 basepairs in length and have 45-55% GC content.

GeneCopoeia’s T7 endonuclease I assay kits, which contain T7E1 and positive controls for both target PCR and indel mismatch cleavage, provide a simple and fast workflow for CRISPR/TALEN validation and screening.

The T7 Endonuclease Detection Assay is a well-known method for detecting genome editing events from CRISPR, Zinc-finger nuclease, and TALEN gene targeting. Originally identified from Escherichia coli bacteriophage, the T7 endonuclease can cleave mismatched heteroduplex DNA, Holliday junctions, branched DNA, and cruciform DNA.