T4 DNA Ligase (5 U/μL) - Thermo Fisher Scientific
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.
T4 DNA Ligase Buffer - Thermo Fisher Scientific
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes (1).
T4 Polynucleotide Kinase (10 U/μL) - Thermo Fisher Scientific
Thermo Scientific™ T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction). The reaction is reversible.
T4 DNA 连接酶 (5 U/μL)
Thermo Scientific T4 DNA 连接酶催化双螺旋 DNA 或 RNA 中并排的 5-磷酸基和 3-羟基端之间磷酸二酯键的形成。 该酶可以修复双螺旋 DNA、RNA 或 DNA/RNA 杂合体中的单链缺口。 其还可以将 DNA 片段与黏性末端或平末端结合，但对单链核酸无活性。
Description: T4 DNA Ligase can be used to join DNA fragments with staggered or blunt ends and to repair nicks in double-stranded DNA having 3'-hydroxyl and 5'-phosphate ends. The enzyme is isolated from E. coli lambda lysogen NM989.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA …
timized for fast and efficient DNA ligation. The efficiency of the fast ligation is analogous to that obtained with T4 DNA Ligase in a standard 1 hour ligation. The ligation reaction mixture can be.
T4 DNA Polymerase (5 U/μL) - Thermo Fisher Scientific
Thermo Scientific T4 DNA Polymerase is a template-dependent DNA polymerase that catalyzes 5'-3' synthesis from primed single-stranded DNA. The enzyme has a 3'-5' exonuclease activity, but lacks 5'-3' exonuclease activity.
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates (forward reaction). The reaction is reversible.
Thermo Scientific™ T4 DNA Ligase (5 U/μL) - Fisher Sci
Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The …