T4 DNA Ligase - NEB
Looking for T4 DNA Ligase alternatives? Try Salt-T4 or Hi-T4 DNA Ligases. GMP-grade reagent also available. Learn more. Catalyzes the formation of a phosphodiester bond between …
Ligation Protocol with T4 DNA Ligase (M0202) - NEB
For blunt ends or single base overhangs, incubate at 16°C overnight or room temperature for 2 hours (alternatively, high concentration T4 DNA Ligase can be used in a 10 minute ligation). …
Salt-T4® DNA Ligase - NEB
A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA …
T4 DNA Ligase Reaction Buffer - NEB
10X reaction buffer supplied with T4 DNA Ligase. A 1X concentration assures optimal enzyme activity.
Use NEBioCalculator to calculate molar ratios. The T4 DNA Ligase Buffer should be thawed and resuspended at room temperature. Gently mix the reaction by pipetting up and down and …
Protocol for Hi-T4™ DNA Ligase (NEB #M2622) | NEB
(Hi-T4 DNA Ligase should be added last. Note that the table shows a ligation using a molar ratio of 1:3 vector to insert for the indicated DNA sizes.) Use NEBiocalculator to calculate molar ratios.
NEB DNA Ligase - New England Biolabs France
Ligation of DNA is a critical step in many modern molecular biology workflows. The sealing of nicks between adjacent residues of a single-strand break on a double-strand substrate and the …
Thaw the T4 DNA Ligase Buffer and resuspend at Room temperature. On ice. T4 DNA Ligase should be added last. Note that the table shows a ligation using a molar ratio of 13 vector to …
DNA Ligases Products | NEB
Browse NEB's high-quality and performance-optimized DNA ligases.
Ligation Protocol WITH T4 DNA Ligase (M0202)
Jan 25, 2015 · Insert DNA (1 kb) 37.5 ng (0.060 pmol) Nuclease-free water to 20 μl. T4 DNA Ligase, 1 μl. Gently mix the reaction by pipetting up and down and microfuge briefly. For …