Oct 13, 2020 · Here we describe a PIK3CA mutation specific nuclease-based enrichment assay, which combined with a low-cost real-time qPCR detection method, enhances assay detection sensitivity from 5% for E542K...

The human T-47D breast cancer cell line was purchased from Thermo Fisher Scientific Inc. T-47D cells were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA) with phenol red and supplemented with 7.5% fetal bovine serum (FBS) plus 100 units/ml penicillin-streptomycin (Sigma-Aldrich).

Aug 28, 2024 · To establish a highly sensitive and specific approach for the detection of circulating PIK3CA H1047R mutation in breast cancer (BC) patients and to investigate the association between the prevalence of PIK3CA H1047R mutation and clinical presentations.

Using this method, we assessed the clinical impact of changes in the frequency and topology of PIK3CA mutation and HER2/ERBB2 amplification within HER2 + breast cancer during neoadjuvant therapy. We found that the two genetic events …

Aug 28, 2024 · The human T-47D breast cancer cell line was purchased from Thermo Fisher Scientific Inc. T-47D cells were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA) with phenol red and supplemented with 7.5% fetal bovine serum (FBS) plus 100 units/ml penicillin-strepto-mycin (Sigma-Aldrich).

Dec 11, 2024 · Dual-luciferase assay confirmed miR-592 binding to the predicted gene PIK3CA. Increasing miR-592 levels decreased the IC50 of TAM, inhibited cell viability, migration, and invasion, and enhanced apoptosis in vitro, which was mimicked by PIK3CA knockdown and reversed by PIK3CA overexpression.

Aug 1, 2019 · Here, we found that continuously activating PIK3CA H1047R conferred human mammary epithelial MCF-10A cells to cellular senescence upon serum-starvation. Similarly, breast cancer T47D and HCC1954 cells harboring H1047R mutation were senescent when cells were deprived of serum.

Jul 7, 2009 · Dysregulation of the phosphatidylinositol 3-kinase (PI3K) signaling pathway occurs frequently in human cancer. PTEN tumor suppressor or PIK3CA oncogene mutations both direct PI3K-dependent tumorigenesis largely through activation of the AKT/PKB kinase.

To evaluate the achievable mutant allele enrichment of PIK3CA mutations, we used genomic DNA from CAL-51, MCF-7, T-47D and EFM-19 cells known to carry the most common PIK3CA hotspot mutations—E542K, E545K, H1047R and H1047L respectively.

Jul 1, 2023 · PI3K, p-AKT, mTOR and HIF-1α were inhibited after treatment with Melatonin + Alpelisib in MDA-MB-453 and T-47D lines. The expression of caspase-3 increased in all groups in MDA-MB-453 and T-47D cells, being the increase more …