Here we investigated the roles of Sox2 and Pax6, transcription factors widely expressed in central nervous system (CNS) progenitors, in mouse NS cells. Conditional deletion of either Sox2 or Pax6 in forebrain-derived NS cells reduced their clonogenicity in a gene dosage-dependent manner.

Feb 9, 2016 · Many Pax6-bound promoters are also targeted by Sox2 and functionally cooperate in gene regulation underlying neuronal specification. Pax6 directly binds and silences genes important for...

Nov 1, 2010 · Pax6 is co-expressed with Sox2 in all these, but is also found in duct/gland cells as well as olfactory neurons that innervate necklace glomeruli. Most of the Sox2/Pax6-positive globose basal cells are actively cycling, but some express the cyclin-dependent kinase inhibitor p27 Kip1, and are presumably mitotically quiescent. Among globose basal ...

In fact, transcription factor pairs that are antagonistic in one cell type or developmental stage may cooperate in other cellular or developmental settings. A case in point is the Sox2/Pax6 pair. Ablation of Sox2 in multipotent optic cup progenitors biases them towards a non-neurogenic ciliary body epithelium fate (Matsushima et al., 2011).

Apr 18, 2019 · Whilst SOX2 interacts with OCT4 under self-renewal conditions, we found that, upon neural differentiation, PAX6, a key neural transcription factor, is upregulated and shows interaction with SOX2. The SOX2-PAX6 complex has different gene regulation pattern from that of SOX2-OCT4 complex.

Apr 1, 2011 · SOX2 is differentially expressed between radial glia and intermediate progenitors. Distinct progenitor populations can be isolated based upon SOX2 expression levels. SOX2-High cells generate larger neurospheres in vitro. SOX2-High cells have increased capacity to generate secondary neurospheres.

May 12, 2016 · These data suggest that Pax6 and Sox2 have counteracting roles in regulating neurogenesis, in which Pax6 accelerates neuronal production, while Sox2 retards it and expands the pool of neuronal progenitors.

Sep 1, 2009 · Here, we investigated the activity of Sox2 in lens induction and its genetic relationship to Pax6 in the mouse. Conditional deletion of Sox2 in the lens placode arrests lens development at the pit stage. As previously shown, conditional deletion of Pax6 in the placode eliminates placodal thickening and lens pit invagination.

May 12, 2016 · These data suggest that Pax6 and Sox2 have counteracting roles in regulating neurogenesis, in which Pax6 accelerates neuronal production, while Sox2 retards it and expands the pool of neuronal progenitors.

Apr 18, 2019 · We demonstrate that knockdown of SOX2 induced hESC differentiation to endoderm-like cells, whereas overexpression of SOX2 in hESCs enhanced their pluripotency under self-renewing culture conditions but promoted their neural differentiation upon replacing the culture to non-self-renewal conditions.