Single primer PCR allows amplification from known to unknown regions in chromosomes, phage, plasmids, large PCR products and other sources of DNA. At sufficiently low stringency, any …

Does any of you have a good resource or protocol for PCR with a single primer? I took my protocol for double-primer RT-PCR but I think I am doing something wrong...

Mar 18, 2019 · We developed a simple protocol, which enabled the retrieval of duplex UMI in multiplex PCR based enrichment and sequencing. Using this protocol and reference materials, …

Jun 30, 2009 · In a widely used method, mutations are generated by PCR using a pair of oligonucleotide primers designed with mismatching nucleotides at the center of the primers. In …

We have explored the possibility of PCR using a single primer. Following reverse transcription (RT) with a gene specific primer, we amplified a specific product using a single internal gene …

This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking …

May 1, 2024 · In this study, we developed a reliable and rapid genome-walking scheme, nominated as single primer site-specific nested PCR (SPN-PCR). This method just requires …

This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking …

Jan 1, 1995 · The basic principle of the single specific primer-polymerase chain reaction (SSP-PCR) procedure has following features: (1) The DNA is digested using two restriction …

Feb 1, 2011 · Here, we compared single- and double-primer amplification and proposed "single-primer PCR correction"; improvements in PCR that eliminate interference caused by single …