For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions.

Monoclonal ANTI-FLAG ® M2, Clone M2 . Produced in Mouse, Affinity Isolated Antibody . F1804 . Storage Temperature −20 °C. Product Description Monoclonal ANTI-FLAG ® M2 is a mouse-derived, affinity-purified IgG1 monoclonal antibody that binds to fusion proteins containing a FLAG ® peptide sequence. 1 The M2 antibody will recognize a FLAG ®

Specificity: Monoclonal ANTI-FLAG® M2 detects a single band of protein on a Western blot from HEK 293 cells over-expressing FLAG ® proteins, crude cell lysate.

Anti-Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus.

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins.

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

flag® 标签在众多应用中得到证实，能够使用抗 flag® 抗体对重组融合蛋白进行卓越的检测和纯化。 FLAG® 和 3x FLAG™ 系统可用于蛋白质表达、蛋白质纯化、蛋白质印迹、免疫细胞化学、免疫沉淀、流式细胞术和下拉分析。

作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。 ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。