Oct 1, 2002 · Using p38alpha mitogen-activated protein kinase (MAPK)-deficient cells, we show that the stress-induced phosphorylation of Ser-727 requires p38alpha MAPK activity, whereas IFN-gamma-stimulated Ser-727 phosphorylation occurs independently of the p38alpha pathway.

Our data suggest that STAT1 is phosphorylated at Ser727 by a stress-activated signaling pathway either through p38 MAPK directly or through an unidentified kinase downstream of p38MAPK. Signal transducers and activators of transcription (STATs) cause rapid transcriptional responses to cytokines (1, 2).

Here we show that p38 mitogen-activated protein kinase (MAPK), an activator of cPLA (2), is essential for both IFN-alpha and IFN-gamma signalling. SB203580, a specific inhibitor of p38, was found to inhibit ISGF3 formation but had no apparent effects on signal transducer and activator of transcription (STAT)1 homodimer formation.

Sep 13, 2002 · Using p38α mitogen-activated protein kinase (MAPK)-deficient cells, we show that the stress-induced phosphorylation of Ser-727 requires p38α MAPK activity, whereas IFN-γ-stimulated Ser-727 phosphorylation occurs independently of the p38α pathway.

Nov 23, 1999 · Our data suggest that STAT1 is phosphorylated at Ser727 by a stress-activated signaling pathway either through p38 MAPK directly or through an unidentified kinase downstream of p38MAPK.

Oct 15, 1999 · Here we show that p38 mitogen‐activated protein kinase (MAPK), an activator of cPLA 2, is essential for both IFN‐α and IFN‐γ signalling. SB203580, a specific inhibitor of p38, was found to inhibit ISGF3 formation but had no apparent effects on signal transducer and activator of transcription (STAT)1 homodimer formation.

Using p38a mitogen- )sequent su Ser-727 phosphorylation (14). activated protein kinase (MAPK)-deficient cells, we show that the STAT1 can be phosphorylated at Ser-727 independently of stress-induced phosphorylation of Ser-727 requires p38a MAPK IF Ns (and phosphorylation at Tyr-701) as well.

Our findings collectively indicate that STAT1 signaling modulates intracellular oxidative stress in activated macrophages through a positive-feedback mechanism involving the p38 MAPK/STAT1/ROS pathway, which is interrupted by caspase activation.

Dec 1, 1999 · Our data suggest that STAT1 is phosphorylated at Ser727 by a stress-activated signaling pathway either through p38 MAPK directly or through an unidentified kinase downstream of p38MAPK. p38MAPK...

R848 and IFN-a activated p38 MAPK are essential for STAT1-S727 phosphorylation and enhanced B cell responses. (A and B) Splenic B cells were stimulated with IFN-a (1000 U/ml, B1I), R848 (1...