ISG15 is an interferon-induced ubiquitin-like modifier which can be conjugated to distinct, but largely unknown, proteins. ISG15 has been implicated in a variety of biological activities, which encompass antiviral defense, immune responses, and pregnancy.

One of the most highly induced genes in the type-I IFN signaling cascade is ISG15 (interferon-stimulated gene 15), which encodes a small ubiquitin-like protein involved in a post-translational modification (PTM) process termed ISGylation. Through this process, ISG15 covalently binds to a wide range of target proteins [5].

Nov 1, 2022 · IRF9 interacts with the signal transducer and activator of transcription 1 (STAT1) and STAT2 and forms the IFN-stimulated gene factor 3 (ISGF3) complex, resulting in ISG15 induction.

Jan 1, 2018 · We found the increased Isg15, Uba7, Ube2l6, Herc6 mRNA levels and profound ISG'ylation in inflammatory microglia. Proteomic analysis of microglial cells depleted of Uba7 revealed the decrease in the level of hundreds proteins and identified Stat1 as one of the protein stabilized by Isg15.

May 16, 2018 · Ubiquitin-like protein ISG15 is an interferon-induced protein that has been implicated as a central player in the host antiviral response. Over the past 15 years, efforts to understand how...

A knockdown of ISG15 upregulated the expression of ISG56, MDA5, CXCL10 and phosphorylated signal transducers and activators of transcription protein 1 (P-STAT1), while a knockdown of ubiquitin-like modifier activating enzyme 7, a key enzyme that conjugates ISG15 to target proteins, did not affect the expression.

ISG15 is an interferon-induced ubiquitin-like modifier which can be conjugated to distinct, but largely unknown, proteins. ISG15 has been implicated in a variety of biological activities, which encompass antiviral defense, immune responses, and pregnancy.

Interferon-stimulated gene 15 (ISG15) is one of the most upregulated genes upon Type I interferon treatment or pathogen infection. Its 17 kDa protein product, ISG15, was the first ubiquitin-like modifier identified, and is similar to a ubiquitin linear dimer.

Oct 12, 2014 · ISG15 deficiency in humans leads to a failure to maintain adequate levels of USP18, triggering an increase in type I interferon production and signalling, and promoting auto-inflammatory disease.

Dec 1, 2023 · ISG15 in its unconjugated (free) form fine-tunes intracellular signaling cascades and, upon extracellular secretion, functions ‘cytokine-like’, contributing to immunomodulation. Viruses from diverse families have evolved tactical strategies to manipulate ISG15-mediated antiviral responses.