Dec 15, 2022 · SspI has a High Fidelity version SspI-HF® (NEB #R3132). High Fidelity (HF®) Restriction Enzymes have 100% activity in CutSmart™ Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.

NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.

Thermo Scientific SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

从 2021 年 4 月开始，NEB 将含有 BSA 的反应缓冲液转换为含有 重组白蛋白（rAlbumin） 的缓冲液，用于限制性内切酶和某些 DNA 修饰酶。 如需了解更多信息，请访问 www.neb.cn/BSA-free。 本酶提供高保真版 SspI-HF®（NEB #R3132）。 高保真（HF®）限制性内切酶在 rCutSmart™ 缓冲液（10X）中具有 100% 活性；统一缓冲液意味着更加直接、简化的样品处理过程。 HF 内切酶还会显著降低星号活性。 所有 HF 内切酶均符合省时酶（Time-Saver™）标准，可在 5-15 分 …

Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang.

NEBuffer™ Set (EcoRI/SspI, DpnII) New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Set contains two 1.25ml vials of each unique NEBuffer: EcoRI/SspI and DpnII.

NEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. We are able to offer >210 restriction enzymes that cut in a single buffer, rCutSmart™ . This improves ease-of-use, especially when performing double digests.

SspI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10141296. Learn more. We are excited to announce that all reaction buffers are now BSA-free.

qPCR DNA Contamination (E. coli Genomic) - A minimum of 20 units of SspI-HF® is screened for the presence of E. coli genomic DNA using SYBR® Green qPCR with primers specific for the E. coli 16S rRNA locus. Results are quantified using a …

Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and …