Sep 1, 2012 · MTF-1 is evolutionarily conserved from insects to humans and is the main activator of metallothionein genes, which encode small cysteine-rich proteins that can scavenge toxic heavy metals and free radicals. MTF-1 has been suggested to act as an intracellular metal sensor but evidence for direct metal sensing was scarce.

Evidence obtained from coimmunoprecipitation assays revealed that exposure of the cells to zinc resulted in the rapid formation of a multiprotein complex containing MTF-1, the histone acetyltransferase p300/CBP, and the transcription factor Sp1.

Jan 9, 2009 · Expression of the prion protein is a vital component for the propagation of prion diseases; thus SP1 and MTF-1 represent new targets in the development of key therapeutics toward modulating the expression of the cellular prion protein and …

Nov 24, 2000 · The Sp1 peptide backbone with the MTF-1 zinc fingers is termed SMS, whereas the MTF-1 peptide backbone with the Sp1 zinc fingers is called MSM. The chimeric DNA constructs were prepared for transcription and translation in TnT lysates and for expression in dko7 (MTF-1−/−) cells.

Jan 9, 2009 · Expression of the prion protein is a vital component for the propagation of prion diseases; thus SP1 and MTF-1 represent new targets in the development of key therapeutics toward modulating the expression of the cellular prion protein and …

Apr 30, 2021 · Here we demonstrated that in brain ischemia induced by transient middle cerebral occlusion (tMCAO), MTF-1 is triggered by a subsequent temporary femoral artery occlusion (FAO) and represents...

Dec 23, 2022 · MT1 and MT2 are considered ubiquitously expressed among the MT isoforms ranging from 1 to 4. These MT1 and MT2 transcriptions are regulated by metal regulatory transcription factor 1 (MTF1) binding to the metal response element (MRE) of the promoter, which is upregulated in response to zinc.

Metal-responsive transcription factor-1 (MTF-1) is a zinc finger protein with a central role in heavy metal homeo-stasis/detoxification. MTF-1 binds to DNA sequence motifs known as metal response elements (MREs) with a core consensus TGCRCNC.

Aug 1, 2023 · Cu up-regulates lipogenesis and induces lipotoxicity disease via Nrf2 activation. Cu affects Cu metabolism and regulates the expression of MTF-1 and SP1. Cu activates Nrf2 through MTF-1 and SP1/Fyn pathway. Excessive Cu intake is harmful to obesity and NAFLD.

Apr 30, 2021 · The aim of this work was to investigate whether the metal responsive transcription factor 1 (MTF-1), an important hypoxia sensitive transcription factor, may (i) interact and regulate NCX1, and (ii) play a role in the neuroprotective effect mediated by RLIP through NCX1 activation.