Platinum qPCR SuperMix for SNP Genotyping is a ready-to-use reaction mix for the amplification and identification of single-nucleotide polymorphisms (SNPs) in genomic DNA using PCR-based SNP genotyping technologies such as fluorescent primers or probes.

Jan 10, 2022 · The presented SNP genotyping results will differ depending on the brand of qPCR instruments and associated computer software. Alternatively, a simple “end-point” approach can be used with a PCR cycler and Microplate Reader with appropriate filters matching the wavelength of the fluorophores used.

How do TaqMan SNP Genotyping Assays work? Like all TaqMan Assays, TaqMan SNP genotyping assays require a double-stranded DNA template, the Taq polymerase enzyme, and two primers—forward and reverse—which are specific to the sequence to be amplified. Unlike gene expression qPCR, SNP detection requires two probes with different fluorescent ...

Here, we present an approach for an economical in-house design of SNP allele-specific qPCR primers/probe sets with a locus-individualized reference system that allows for the accurate quantification of the respective informative locus using a simple ΔΔCt method.

Jan 31, 2017 · Next-generation sequencing (NGS) has led to a dramatic increase in identified SNPs. SNPs can pose a problem when they underlie primer or probe sequences used in PCR/qPCR. Learn what effect they can have and how you …

Wide applicability analysis of UPIP-qPCR in SNP genotyping. Based on UPIP-qPCR, we designed primers (Table S2) and genotyped 13 different SNPs. These SNPs covered all SNP mutation types.

Jul 26, 2024 · One of the most potent and empowering tools for SNP detection is the Real-Time Polymerase Chain Reaction (qPCR). qPCR, a technique used to amplify a specific DNA segment, is the ideal method for SNP detection. This blog post will explore how qPCR empowers us with its sensitivity in SNP detection.

Feb 15, 2019 · The resulting method, called double-mismatch allele-specific qPCR (DMAS-qPCR), was successfully validated using 12 SNPs and 15 clinically relevant somatic mutations on 48 cancer...

LGC, Biosearch Technologies offers BHQplus Probes for SNP genotyping. BHQplus Probes incorporate a duplex-stabilising chemistry that permits the design of shorter oligonucleotides while maintaining optimal probe melting temperature (Tm) for real-time PCR.

You can easily design probes and primers for gene expression and SNP genotyping using our free, online qPCR assay design program called RealTimeDesign software.