Stress-mediated degradation of SIRT3. (A) Left, turnover kinetics of SIRT3. 293T cells were incubated with 50 μg/ml cycloheximide (CHX) for the indicated times. Degradations of the endogenous full-length (FL) and short (Short) forms of SIRT3 were monitored by Western blotting using anti-SIRT3.

Jun 1, 2023 · We found that baicalin could mimic the effect of MG-132 to inhibit CHX-induced degradation of SIRT3 and Ang II-induced proteasomal chymotrypsin-like activity, which was consistent with previous studies reporting that baicalin was a proteasome inhibitor targeting chymotrypsin-like activity.

SIRT3 is a NAD+-dependent deacetylase that regulates the function of numerous mitochondrial proteins with roles in metabolism, oxidative stress, and cell survival. It is emerging as an instrumental regulator of the mitochondrial adaptive responses ...

Dec 22, 2016 · SIRT5 deacetylates STAT3, thereby inhibiting its function in mitochondrial pyruvate metabolism. In the A549 lung cancer cell line, constitutively acetylated STAT3 localizes to mitochondria, where...

Recent studies by two independent laboratories have identified SIRT3 as a tumor suppressor that inhibits the expression of hypoxia-inducible factor 1α (23, 24). SIRT3 also regulates the production of reactive oxygen species by deacetylating superoxide dismutase (25, 26).

Oct 26, 2018 · Serine hydroxymethyltransferase 2 (SHMT2) converts serine to glycine in mitochondria and is upregulated in a variety of cancers. Here the authors show that acetylation of the lysine-95 (K95) residue negatively regulates SHMT2 expression and activity and is deacetylated by SIRT3 in colorectal cancer.

Feb 4, 2023 · We found that intestinal epithelial cell-specific ablation of Sirt3 promotes primary tumor growth via stabilizing mitochondrial LONP1. Notably, we newly identified that Sirt3 deacetylates human oncogene LONP1 at N terminal residue lysine 145 (K145).

Jul 7, 2016 · We showed that Sirt3 is important for the clearance of the damaged mitochondria through activating mitophagy, and silencing of Sirt3 expression can enhance the sensitivity of tumor cells to stress by inhibiting autophagy and promoting apoptosis.

May 7, 2021 · CRISPR/Cas9-mediated depletion of SIRT3 led to compromised nuclear integrity, loss of heterochromatin and accelerated senescence in hMSCs. Further analysis indicated that SIRT3 interacted with nuclear envelope proteins and heterochromatin-associated proteins.

Mar 4, 2010 · Here we report that SIRT3 expression is upregulated during fasting in liver and brown adipose tissues. During fasting, livers from mice lacking SIRT3 had higher levels of fatty-acid oxidation...