Aug 29, 2022 · Fos expression was induced in MLR → RVLM neurons by ChR2-mediated optogenetic activation of their cell bodies under anesthesia, and this induced Fos expression was suppressed by simultaneous...

A lentivirus that expresses channelrhodopsin-2 (ChR2) under the control of the catecholaminergic neuron-preferring promoter PRSx8 was introduced into the rostral ventrolateral medulla (RVLM). After 2–3 weeks, ChR2 was largely confined to Phox2b-expressing neurons (89%).

Following injections of AAV2–ChR2–mCherry (~400 nL) into the left RVLM, an implantable 200-lm-diameter fiber optic was placed with its tip 400 lm above the injection site (~4.7 mm ventral to the dorsal surface of the brain), and the fiber optic was secured to …

Methods: A Cre-dependent vector expressing channelrhodopsin 2 (ChR2) fused with enhanced yellow fluorescent protein or mCherry was injected into the RVLM of tyrosine hydroxylase (TH)-Cre rats. The response of ChR2-transduced neurons to light was examined in anesthetized rats.

Feb 2, 2013 · Selective expression of ChR2-mCherry by RVLM-CA neurons was achieved by injecting Cre-dependent vector AAV2-EF1α-DIO-ChR2-mCherry unilaterally into the brainstem of dopamine-β-hydroxylase Cre/0 mice. Photostimulation of RVLM-CA neurons increased breathing in anesthetized and conscious mice.

ChR2-mCherry was expressed by similar numbers of rostral ventrolateral medulla (RVLM) neurons in each strain (~400 neurons), with identical selectivity for catecholaminergic neurons (90-99% colocalisation with tyrosine hydroxylase).

Nov 1, 2010 · Measurements and main results: The RVLM contained 600 to 900 ChR2-transfected neurons (63% C1, 37% ccRTN). RVLM photostimulation significantly increased breathing rate (+42%), tidal volume (21%), minute volume (68%), and peak expiratory flow (48%).

Dec 1, 2009 · A lentivirus that expresses channelrhodopsin-2 (ChR2) under the control of the catecholaminergic neuron-preferring promoter PRSx8 was introduced into the rostral ventrolateral medulla (RVLM). After 2-3 weeks, ChR2 was largely confined to …

Dec 1, 2014 · We labeled RVLM neurons with a lentiviral vector that expresses the fusion protein ChR2-mCherry under the control of the catecholaminergic neuron-selective promoter PRSx8 and obtained light and ultrastructural evidence that these neurons innervate the orexin cells.

Dec 1, 2014 · Methods: A Cre-dependent vector expressing channelrhodopsin 2 (ChR2) fused with enhanced yellow fluorescent protein or mCherry was injected into the RVLM of tyrosine hydroxylase (TH)-Cre rats. The response of ChR2-transduced neurons to light was examined in anesthetized rats.