Apr 1, 2015 · In the Tet-Off expression system, a tetracycline-controlled transactivator protein (tTA), which is composed of the Tet repressor DNA binding protein (TetR) from the Tc …

Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of …

The reverse-tTA (rtTA) variant exhibits a reverse phenotype and does not bind tetO in the absence of an effector. Binding of dox triggers a conformational switch in rtTA, which allows …

Apr 1, 2002 · In combination with rtTA, tTS actively suppresses background expression or “leakiness” without impeding the inducibility of the target gene, providing a true “On/Off” …

四环素 (Tet)诱导调控表达系统，是在大肠杆菌 Tn10转座子 中特异的 Tet抗性操纵子 基础上建立起来的一种用于诱导基因表达的调控系统。 目前，此系统已被广泛应用于基因功能和基因治疗 …

May 25, 2006 · Upon dox administration, rtTA activates transcription from the LTR-tetO promoter, resulting in expression of the viral proteins and subsequent viral replication.

四环素 (Tetracycline, Tet)调控基因表达系统便可以做到对基因表达的精准调控，今天小编便来介绍这一神奇的基因开关。 四环素调控基因表达系统是以大肠杆菌 Tn10转座子 上Tet抗性操纵子 …

These double mutant Rosa26-rtTA::Col1a1-tetO-H2B-mCherry mice have widespread expression of the optimized form of reverse tetracycline-controlled transactivator (rtTA-M2) protein …

This transgenic mouse line was generated by co-injection of two transgenes, a “reverse” tetracycline-controlled transactivator (rtTA) directed by a rat cardiac troponin T (Tnnt2) …

Jun 14, 2022 · 1992年Goseen等人成功的利用原核基因调控元件构建了四环素（tetracycline,Tet）真核细胞基因调控表达系统。 目前，此系统已被广泛应用于基因功能和基 …