Jun 3, 2021 · What is ROX dye? ROX dye is an inert fluorescent dye that can be added as one of the components in a qPCR master mix. Unlike reporter dyes such as SYBR™ Green or FAM™ dye, the fluorescence of ROX dye is not affected by amplification of the PCR product.

ROX is a fluorescent passive reference dye with a level of fluorescence that does not change during the PCR process. Normalization of qPCR data using ROX reduces the influence of well-to-well variability such as uneven illumination, slight variation in optics, and differences in the amount of condensation.

ROX Reference Dye: Normalizes non-PCR-related fluctuations in fluorescence • Provides a stable baseline for multiplex quantitative PCR and RT-PCR • Uses existing ROX spectral settings, eliminating the need to re-calibrate the thermal cycler

ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.

ROX (carboxy-X-rhodamine) is used as a passive reference dye in ROX-dependent real-time PCR systems to normalize for differences in fluorescence levels that can occur due mainly to optical path variations among wells.

May 20, 2019 · ROX, also known as carboxyrhodamine, is an inert fluorescent dye that can be added to the qPCR master mix. Unlike reporter dyes in qPCR (e.g. SYBR Green), ROX is referred to as a passive dye, since its fluorescence signal is not influenced by the PCR reactions.

Aug 4, 2023 · ROX reference dye is essential to the accuracy and reproducibility of qPCR reactions performed on ROX-dependent PCR cyclers (e.g., the Applied Biosystems® instruments).

osystemsTM ROXTM dye is a proprietary passive reference dye introduced in the 1990s. ROX dye is inert and does not interfer. with the fluorescence emission of the reporter dyes commonly used in real-time PCR. Today all Applied BiosystemsTM real-time PCR systems are designed to take advantage of.

ROX Reference Dye provides users of the ABI PRISM® 7700 an effective alternative for normalization of fluorescent reporter signal in real-time quantitative PCR or RT-PCR without modifying the instrument’s default analysis parameters. ROX Reference Dye …

ROX Passive Reference Dye is specially formulated for use on Applied BiosystemsTM (ABI) and StratageneTM real-time PCR instruments. This inert dye, whose fluorescence does not change during the reaction, may be added to quantitative, real-time PCR reactions to normalize the well-to-well differences that may occur due to artifacts such as ...