Feb 25, 2022 · In this minireview, we introduce the basic structure and function of HMGB1 and focus on the regulatory mechanism of HMGB1 secretion and release. Understanding these topics may help us develop...

Reactive oxygen species (ROS) level was evaluated to estimate the translocation mechanism of HMGB1. The role of secreted HMGB1 was evaluated by ELISA assay.

ROS induces HMGB1 oxidation and cytosolic translocation from the nucleus. Cytoplasmic HMGB1 binds to Beclin 1 using an intramolecular disulfide bridge (Cys23 and Cys45) to enhance autophagic flux (37, 124, 125).

Sep 14, 2016 · We suggest that HMGB1 is secreted in hypoxic condition via ROS-dependent mechanism and secreted HMGB1 participates in IL-8 upregulation mediating inflammatory response.

HMGB1-mediated pyroptosis in DDP/VP16-treated cells regulated ROS generation and ERK1/2 phosphorylation in vitro and in vivo. The discovery of HMGB1 as a critical regulator of pyroptosis has provided new insights for therapeutic interventions in neuroblastoma.

Mar 1, 2025 · HMGB1 is secreted extracellularly in its monomeric form and subsequently dimerized by ROS and PAMPs. Cys 106 is crucial for antiparallel dimerization of HMGB1 via disulfide bond formation. Di-HMGB1 increases binding affinity for TLR2 and TLR4, thereby enhancing pro-inflammatory signaling.

High-mobility group box 1 (HMGB1) protein, a chromatin-binding nuclear protein and damage-associated molecular pattern molecule, is integral to oxidative stress and downstream apoptosis or survival. Accumulation of HMGB1 at sites of oxidative DNA damage can lead to …

Feb 15, 2023 · Our study suggests that HMGB1 is a novel regulator of pyroptosis via the ROS/ERK1/2/caspase-3/GSDME pathway and a potential drug target for therapeutic interventions in neuroblastoma. Keywords: GSDME; HMGB1; neuroblastoma; pyroptosis.

Feb 5, 2021 · Endothelial-specific deletion of HMGB1 (HMGB1ECKO) was generated and Akt/eNOS signaling, reactive oxygen species (ROS) production, endothelium dependent relaxation (EDR), and angiogenesis were determined in vitro and in vivo.

Apr 1, 2022 · To overcome the shortcomings of GA delivery and to improve the efficacy of cerebral ischemia therapy, herein, we designed reactive oxygen species (ROS) responsive polymer-drug conjugate nanoparticles (DGA) to manipulate microglia polarization by suppressing the translocation of nuclear HMGB1.