In summary, a role of GLUT1 in regulation of ROS levels has been elucidated by means of GLUT1 inhibitors that increase ROS and expression of GLUT1 with mutations at an ATM target site that prevent (S490A) or mimic (S490D) phosphorylation and increase or …

Moreover, GLUT1 contributes to ROS scavenging by mediating the transport of dehydroascorbic acid (DHA), the oxidized form of vitamin C, which is recycled back to vitamin C inside the cell (Rumsey et al. 1997).

Mar 25, 2014 · We found that the GLUT1 inhibitors fasentin and phloretin increased the ROS levels induced by antimycin A and the superoxide generator pyrogallol. However, indinavir, which inhibits GLUT4 but not GLUT1, had no effect on ROS levels.

Jul 27, 2023 · Mechanistically, Glut1 inactivation causes metabolic rewiring toward oxidative phosphorylation, which generates an excessive amount of reactive oxygen species (ROS). Accumulated ROS potentiate tumor cell death mediated by tumor necrosis factor alpha (TNF-α) in a caspase-8- and Fadd-dependent manner.

May 30, 2021 · GLUT-1 inhibition suppressed not only the ROS levels but also the cell proliferation in KFs. In summary, the GLUT-1-dependent glycolysis and ROS production mediated fibroblast proliferation in keloids. GLUT1 might be a potential target for metabolic reprogramming to …

We found that the oxidized form of vitamin C, dehydroascorbic acid (DHA), enters mitochondria via facilitative glucose transporter 1 (Glut1) and accumulates mitochondrially as ascorbic acid (mtAA).

Jun 20, 2018 · Our data showed that aspirin significantly decreased the levels of reactive oxygen species (ROS) and glucose consumption in hepatoma cells. Interestingly, we identified that GLUT1 and HIF1α could...

Mar 25, 2014 · GLUT1-mediated transport would cause increased reactive oxygen species (ROS) levels in L6 myoblasts, while conditions that would increase GLUT1-mediated transport would result in decreased...

Mar 14, 2014 · Through radiotracer, metabolomics, bioenergetics, and expression analysis studies, we have demonstrated that elevated GLUT1-driven glucose metabolism drives reactive oxygen species (ROS) production and expression of proinflammatory mediators.

Nov 1, 2004 · As the amount of Glut1 that is distributed between the intracellular and cell surface compartments represents the balance between exocytosis and endocytosis, we can speculate that ROS might be involved in Glut1 vesicle trafficking in B1647 cells.