A ribonucleotide tri-phosphate (rNTP) is composed of a ribose sugar, 3 phosphate groups attached via diester bonds to the 5' oxygen on the ribose and a nitrogenous base attached to the 1' carbon on the ribose. rNTP's are also referred to as NTPs while the deoxyribose version is referred to as dNTPs.

This work explored the origin of discrimination between rNTP and to dNTP by DNA/RNA polymerases, using systematic calculations of the change in the transition state (TS) binding free energy in RB69 DNA polymerase and T7 RNA polymerase.

May 24, 2013 · The current study reconstitutes the Escherichia coli replisome and examines the cost of rNTP/dNTP nucleotide pool imbalance on the frequency of rNMP incorporation and the rate of fork progression.

Aug 2, 2016 · Understanding the origin of discrimination between rNTP and dNTP by DNA/RNA polymerases is important both for gaining fundamental knowledge on the corresponding systems and for advancing the design of specific drugs.

Understanding the origin of discrimination between rNTP and dNTP by DNA/RNA polymerases is important both for gaining fundamental knowledge on the corresponding systems and for advancing the design of specific drugs.

Here, we fill the methodological gap by presenting a simple non-radioactive microplate assay for the quantification of dNTPs with a minimum requirement of 4–12 mg of biopsy material.

Aug 6, 2013 · The concentration of ribonucleoside triphosphates (rNTPs) in cells is far greater than the concentration of deoxyribonucleoside triphosphates (dNTPs), and this pool imbalance presents a challenge for DNA polymerases (Pols) to select their proper substrate.

Comparison of intracellular dNTP and rNTP concentrations with the enzyme kinetics of cellular and viral polymerases reveals a dynamic relationship between enzyme kinetics and physiological substrate availability.

In this study, we investigated the ability of hpol η to incorporate ribonucleotides into DNA and crystallized hpol η with incoming rNTPs opposite both undamaged DNA and an 8-oxodG lesion.

Feb 4, 2018 · DNA polymerase: It actively discriminates against rNTPs via a “ STERIC gate ” formed by the Glu and Phe side chains, which excludes 2′-OH group of rNTP. But because of high concentration of rNTPs, they still get incorporated into DNA by Pol δ and ε. rNTP incorporation rate is 1 in 1000 dNTP.