Oct 1, 2018 · For example, REST4, which was first described in rat as a group of REST isoforms (Palm et al., 1998), is predicted by multiple mRNA variants (e.g., JX896958, JX896971, and JX896983) with E 3 followed by variable exons that introduce a pre-mature stop codon.

REST is highly conserved in mammals, and there are various mRNA alternative splice variants predictive of multiple protein isoforms. Studies showed that REST might produce at least 45 mRNA variants encoding REST4, REST1, REST C, and small peptides [3].

May 19, 2017 · The splicing event ∆E 3, which is common in human and non‐human primates, results in REST ∆ protein isoform(s) missing ZF ‐5 critical for nuclear targeting and therefore modulates nuclear REST levels and genome function.

Sep 2, 2011 · In this study, we show that nSR100 directly activates the inclusion of a neural-specific exon in REST transcripts leading to the production of a truncated isoform of REST (REST4) (Palm et al., 1998), which lacks critical domains required for REST-mediated transcriptional silencing of target genes.

Apr 1, 2019 · Alternative splicing of REST mRNA generates various REST isoforms which lack key regulatory domains, e.g. ZF5 and/or NLS which are thought to be required for nuclear localisation. Known isoform REST4 is read by exon E2-E3 and only contains the N-terminal repressor domain and ZFs 1–5.

Jun 8, 2015 · Suppression or loss of REST function may also result from alternative splicing. An alternatively spliced form of REST, called REST4, has been identified in several cancer types that do not...

Nov 4, 2010 · An alternatively spliced isoform, REST4, was found to be produced exclusively in post-mitotic neurons, and lacks a key repressor domain [16,24]. REST4 is thought to facilitate silencing of REST repression; however, these mechanisms are not yet clear.

Jan 11, 2002 · In neuronal cells, a truncated, neuronal-specific REST/NRSF isoform, REST4, has been found but little is known about its function. To address this, we investigated the effect of REST/NRSF and REST4 on the activity-dependent activation of BDNF gene promoter I (BDNFp-I) using cultured rat cortical neurons.

May 1, 2010 · Isoform 3, also called REST4 or sNRSF, is expressed in neuroblastoma and SCLC; it lacks RD2 and has a truncated DNA binding domain, but retains zinc fingers 1-5 and nuclear localization. Isoform 4 has selective deletion of zinc finger 5.

Overexpressing SRRM4, known for regulating ME inclusion in mRNAs critical for neural differentiation, in human HEK293 cells resulted in the biogenesis of over 2000 novel ME-circRNAs, including ME-circEIF4G3, and changes in the abundance of many canonical circRNAs, including circSETDB2 and circLRBA.