Oct 24, 2024 · Rodent Blood Collection. Contact an RARC trainer for training or guidance on using these blood collection techniques. Factors to consider when choosing the blood withdrawal technique: species of rodent to be bled; size/weight of the animal to be bled; quality of the sample required (sterility, tissue fluid contamination, etc.) quantity of blood ...

The approximate total blood volume of a rat is 55-70 ml/kg (mean 64ml/kg) of body weight. The total blood volume for a 300 g rat this is approximately 17-21 ml. • For optimal health, blood draws should be limited to the lower end of the range. Maximum blood volumes should be taken only from healthy animals.

Various regulatory agencies and guidelines have restricted the use of animals and the techniques used for blood collection in laboratory animals. This article deals with the approved blood collection techniques for laboratory animals like rodents, lagomorphs and nonrodents.

Understanding the blood physiology of rats is essential for veterinarians who treat pet rats or laboratory animals. Blood tests are crucial for diagnosing and monitoring diseases, as well as for evaluating the health status of these animals.

Collecting blood from a small rodent is perhaps the greatest barrier to obtain laboratory data for these pets. Both the source and method of blood collection can affect blood parameters. Increases in blood hormone and glucose levels are directly related to stressful methods of …

For information on determining appropriate blood volumes and frequency, review the NIH ARAC Guidelines for Survival Bleeding of Rodents. The Mandibular Vein is located under the jawbone of the mouse (as shown in the first picture). This technique …

These guidelines have been developed to assist investigators and National Institutes of Health (NIH) Institute/Center (IC) Animal Care and Use Committees (ACUC) in their choice and application of survival rodent bleeding techniques. The guidelines are based on peer-reviewed publications1-9 and data and experience accumulated at NIH.

When designing an experiment that will require blood collection from rodents, several basic parameters such as the following, should be carefully considered. • Survival blood collection versus terminal blood collection • Species of animal • …

Guidelines for Blood Collection in Mice and Rats This document provides direction and guidance on appropriate blood collection methods and volumes for animals used in research at the AECOM.

Total blood volume (TBV) of a mouse is approximately 8% of body weight or 80 µl/g. Total blood volume for a rat is approximately 6% of body weight or 60 µl/g. Recommended Methods and General Guidelines for Blood Collection in Mice